Effect of the dam strain on the spontaneous incidence of cleft lip and palate and intrauterine growth of CL/Fr mouse fetuses

Kazuaki Nonaka, Yasunori Sasaki, Deborah A. Martin, Minoru Nakata

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Purpose: The effects of dam strain on the spontaneous incidence of the cleft lip and palate (CLP) and the intrauterine growth of transferred CLP-susceptible CL/Fr embryos were examined with an embryo transfer technique. The CL/Fr strain of embryos at the early blastocyst stage was transferred to the same dam strain and also to the CLP-resistant C57BL dam strain. A laparotomy was done on the 18th gestational day at which time the number of fetuses, the resorption sites and the fetus weight were recorded. Each fetus was checked for the presence of CLP. Five criteria to assess the reproduction and fertility as well as the fetus weight were then compared between both dam strains. Results: The dam pregnancy rate and the fetus survival rate in the CL/Fr dam strain were both significantly lower than those in the C57BL dam strain. The resorption rate in the CL/Fr dam strain was significantly higher than that in the C57BL dam strain. The spontaneous incidence rate of CLP in the CL/Fr dam strain was also significantly higher than that in the C57BL dam strain. The fetus weight of the CL/Fr fetuses developed in the CL/Fr dam strain was significantly lighter than that of the CL/Fr fetuses developed in the C57BL dam strain. Conclusion: The results indicated that the CLP-susceptible CL/Fr dam strain provided a less favorable uterine environment for the implantation, survival and intrauterine growth of the transferred CL/Fr embryos and also caused a higher spontaneous incidence rate of CLP. Thus, it can be concluded that the effect of the dam strain appears to play an important role on the spontaneous incidence of CLP and the intrauterine growth of the CL/ Fr strain embryos transferred to both CL/Fr and C57BL dam strains.

Original languageEnglish
Pages (from-to)447-452
Number of pages6
JournalJournal of Assisted Reproduction and Genetics
Volume12
Issue number7
DOIs
Publication statusPublished - Aug 1 1995

Fingerprint

Cleft Lip
Cleft Palate
Fetus
Incidence
Growth
Embryonic Structures
Weights and Measures
Embryo Transfer
Blastocyst
Pregnancy Rate
Laparotomy
Reproduction
Fertility
Survival Rate

All Science Journal Classification (ASJC) codes

  • Reproductive Medicine
  • Genetics
  • Obstetrics and Gynaecology
  • Developmental Biology
  • Genetics(clinical)

Cite this

Effect of the dam strain on the spontaneous incidence of cleft lip and palate and intrauterine growth of CL/Fr mouse fetuses. / Nonaka, Kazuaki; Sasaki, Yasunori; Martin, Deborah A.; Nakata, Minoru.

In: Journal of Assisted Reproduction and Genetics, Vol. 12, No. 7, 01.08.1995, p. 447-452.

Research output: Contribution to journalArticle

Nonaka, Kazuaki ; Sasaki, Yasunori ; Martin, Deborah A. ; Nakata, Minoru. / Effect of the dam strain on the spontaneous incidence of cleft lip and palate and intrauterine growth of CL/Fr mouse fetuses. In: Journal of Assisted Reproduction and Genetics. 1995 ; Vol. 12, No. 7. pp. 447-452.
@article{5d439baad83b4cabb64ed88b36c84330,
title = "Effect of the dam strain on the spontaneous incidence of cleft lip and palate and intrauterine growth of CL/Fr mouse fetuses",
abstract = "Purpose: The effects of dam strain on the spontaneous incidence of the cleft lip and palate (CLP) and the intrauterine growth of transferred CLP-susceptible CL/Fr embryos were examined with an embryo transfer technique. The CL/Fr strain of embryos at the early blastocyst stage was transferred to the same dam strain and also to the CLP-resistant C57BL dam strain. A laparotomy was done on the 18th gestational day at which time the number of fetuses, the resorption sites and the fetus weight were recorded. Each fetus was checked for the presence of CLP. Five criteria to assess the reproduction and fertility as well as the fetus weight were then compared between both dam strains. Results: The dam pregnancy rate and the fetus survival rate in the CL/Fr dam strain were both significantly lower than those in the C57BL dam strain. The resorption rate in the CL/Fr dam strain was significantly higher than that in the C57BL dam strain. The spontaneous incidence rate of CLP in the CL/Fr dam strain was also significantly higher than that in the C57BL dam strain. The fetus weight of the CL/Fr fetuses developed in the CL/Fr dam strain was significantly lighter than that of the CL/Fr fetuses developed in the C57BL dam strain. Conclusion: The results indicated that the CLP-susceptible CL/Fr dam strain provided a less favorable uterine environment for the implantation, survival and intrauterine growth of the transferred CL/Fr embryos and also caused a higher spontaneous incidence rate of CLP. Thus, it can be concluded that the effect of the dam strain appears to play an important role on the spontaneous incidence of CLP and the intrauterine growth of the CL/ Fr strain embryos transferred to both CL/Fr and C57BL dam strains.",
author = "Kazuaki Nonaka and Yasunori Sasaki and Martin, {Deborah A.} and Minoru Nakata",
year = "1995",
month = "8",
day = "1",
doi = "10.1007/BF02211146",
language = "English",
volume = "12",
pages = "447--452",
journal = "Journal of Assisted Reproduction and Genetics",
issn = "1058-0468",
publisher = "Springer New York",
number = "7",

}

TY - JOUR

T1 - Effect of the dam strain on the spontaneous incidence of cleft lip and palate and intrauterine growth of CL/Fr mouse fetuses

AU - Nonaka, Kazuaki

AU - Sasaki, Yasunori

AU - Martin, Deborah A.

AU - Nakata, Minoru

PY - 1995/8/1

Y1 - 1995/8/1

N2 - Purpose: The effects of dam strain on the spontaneous incidence of the cleft lip and palate (CLP) and the intrauterine growth of transferred CLP-susceptible CL/Fr embryos were examined with an embryo transfer technique. The CL/Fr strain of embryos at the early blastocyst stage was transferred to the same dam strain and also to the CLP-resistant C57BL dam strain. A laparotomy was done on the 18th gestational day at which time the number of fetuses, the resorption sites and the fetus weight were recorded. Each fetus was checked for the presence of CLP. Five criteria to assess the reproduction and fertility as well as the fetus weight were then compared between both dam strains. Results: The dam pregnancy rate and the fetus survival rate in the CL/Fr dam strain were both significantly lower than those in the C57BL dam strain. The resorption rate in the CL/Fr dam strain was significantly higher than that in the C57BL dam strain. The spontaneous incidence rate of CLP in the CL/Fr dam strain was also significantly higher than that in the C57BL dam strain. The fetus weight of the CL/Fr fetuses developed in the CL/Fr dam strain was significantly lighter than that of the CL/Fr fetuses developed in the C57BL dam strain. Conclusion: The results indicated that the CLP-susceptible CL/Fr dam strain provided a less favorable uterine environment for the implantation, survival and intrauterine growth of the transferred CL/Fr embryos and also caused a higher spontaneous incidence rate of CLP. Thus, it can be concluded that the effect of the dam strain appears to play an important role on the spontaneous incidence of CLP and the intrauterine growth of the CL/ Fr strain embryos transferred to both CL/Fr and C57BL dam strains.

AB - Purpose: The effects of dam strain on the spontaneous incidence of the cleft lip and palate (CLP) and the intrauterine growth of transferred CLP-susceptible CL/Fr embryos were examined with an embryo transfer technique. The CL/Fr strain of embryos at the early blastocyst stage was transferred to the same dam strain and also to the CLP-resistant C57BL dam strain. A laparotomy was done on the 18th gestational day at which time the number of fetuses, the resorption sites and the fetus weight were recorded. Each fetus was checked for the presence of CLP. Five criteria to assess the reproduction and fertility as well as the fetus weight were then compared between both dam strains. Results: The dam pregnancy rate and the fetus survival rate in the CL/Fr dam strain were both significantly lower than those in the C57BL dam strain. The resorption rate in the CL/Fr dam strain was significantly higher than that in the C57BL dam strain. The spontaneous incidence rate of CLP in the CL/Fr dam strain was also significantly higher than that in the C57BL dam strain. The fetus weight of the CL/Fr fetuses developed in the CL/Fr dam strain was significantly lighter than that of the CL/Fr fetuses developed in the C57BL dam strain. Conclusion: The results indicated that the CLP-susceptible CL/Fr dam strain provided a less favorable uterine environment for the implantation, survival and intrauterine growth of the transferred CL/Fr embryos and also caused a higher spontaneous incidence rate of CLP. Thus, it can be concluded that the effect of the dam strain appears to play an important role on the spontaneous incidence of CLP and the intrauterine growth of the CL/ Fr strain embryos transferred to both CL/Fr and C57BL dam strains.

UR - http://www.scopus.com/inward/record.url?scp=0029093666&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029093666&partnerID=8YFLogxK

U2 - 10.1007/BF02211146

DO - 10.1007/BF02211146

M3 - Article

C2 - 8574073

AN - SCOPUS:0029093666

VL - 12

SP - 447

EP - 452

JO - Journal of Assisted Reproduction and Genetics

JF - Journal of Assisted Reproduction and Genetics

SN - 1058-0468

IS - 7

ER -