One group of 60 carp (ca 100 g each) were fed with a diet containing polychlorinated biphenyl (PCB) (1 mg PCB/kg body weight/day) for 2 weeks to induce drug-metabolizing enzymes. A second group of 60 carp were fed a PCB-free diet as a control. After pretreatment, oxolinic acid (OA) was administered in feed to PCB-treated and control fish in a single dose of 20 mg OA/kg body weight. The peak concentrations of OA in the blood, liver and muscle of PCB-treated fish were lower than half of those in the control. Furthermore, the duration times of OA in the liver and blood of PCB-treated fish were also shortened compared with those in the control. In another test in vitro with hepatic microsomes, the produced amount of dihydroxy-OA in PCB-treated fish increased more than 30-fold compared with that in controls. These facts suggest that the induction of the drug-metabolizing enzyme activity by pollutants such as PCB, will result in large differences in the duration of drugs in fish, when the drugs accept enzymatic reactions.
All Science Journal Classification (ASJC) codes
- Aquatic Science