Effect of Toki-Shakuyaku-San on acetylcholine level and blood flow in dorsal hippocampus of intact and twice-repeated ischemic rats

Izzettin Hatip-Al-Khatib, Funda Bölukbaşi Hatip, Yoshitaka Yoshimitsu, Katsunori Iwasaki, Nobuaki Egashira, An Xin Liu, Kenichi Mishima, Michihiro Fujiwara

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The effects of Toki-shakuyaku-san (TSS) ingredients on acetylcholine (ACh) release in the dorsal hippocampus (DH) were investigated in intact and twice-repeated ischemic rats using in vivo microdialysis-HPLC. Moreover, the effect of TSS on blood flow was investigated in intact rats using laser Doppler flowmetry. TSS at 300 mg/kg p.o. increased ACh and blood flow after 40 min in intact rats. TSS also increased ACh in ischemic rats but to a lesser extent than in intact rats. These results could suggest that TSS-increased ACh and blood flow in DH may contribute in the cognition improving property of TSS.

Original languageEnglish
Pages (from-to)291-294
Number of pages4
JournalPhytotherapy Research
Issue number3
Publication statusPublished - Mar 1 2007
Externally publishedYes


All Science Journal Classification (ASJC) codes

  • Drug Discovery
  • Organic Chemistry
  • Pharmacology

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