Effect of ultrasound on DNA polymerase reactions: Monitoring on a 27-MHz quartz crystal microbalance

Yu Hoshino, Takayoshi Kawasaki, Yoshio Okahata

Research output: Contribution to journalArticlepeer-review

36 Citations (Scopus)

Abstract

Effects of ultrasound irradiation on DNA polymerase (Klenow fragment, KF) reactions were studied on the template/primer DNA-immobilized quartz crystal microbalance (QCM). Under ultrasound irradiation, binding of KF to the DNA was suppressed due to the decrease of the binding rate constant (k1) and the increase of the dissociation rate constant (k-1). The catalytic elongation rate (kcat) was increased, but the stability of the KF/DNA/monomer ternary complex (Km) was decreased by the ultrasound irradiation. Ultrasound effects are discussed in correlation with the conformation changes of domain structures in KF.

Original languageEnglish
Pages (from-to)682-685
Number of pages4
JournalBiomacromolecules
Volume7
Issue number3
DOIs
Publication statusPublished - Mar 1 2006
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

Fingerprint Dive into the research topics of 'Effect of ultrasound on DNA polymerase reactions: Monitoring on a 27-MHz quartz crystal microbalance'. Together they form a unique fingerprint.

Cite this