Effect of vitamin C on mechanical property of the regenerated-cartilage tissue

S. Omata, T. Shimizu, Y. Sawae, T. Murakami

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Tissue engineering has become a promising option toward the treatment of osteoarthritis, allowing researchers to produce functional replacements for diseased cartilage. The mechanical behavior of articular cartilage depends on an extracellular matrix (ECM), i.e., type II collagen and proteoglycans. The collagen fibers play an important role to establish the mechanical strength in the cartilage tissue. Thus we focused the influence of L-ascorbic acid (AsA, as an acidic form) and ascorbic acid 2-phosphate (A2P, as a non-acidic form), as different concentrations in culture medium on mechanical property of the regenerated cartilage tissue because the collagen synthesis needs AsA. By increasing AsA concentration in culture medium, the result indicated that the tangent modulus of the cultured constructs was significantly increased and the synthesized collagen fibrils were spread out spatially in the immunofluorescence images. This study strongly suggests, therefore, that mechanical property of regenerated-cartilage tissue depended on interconnecting among cells by the ECMs, mainly by the collagen network. In contrast, significant damage by cellular toxicity was found in the high AsA concentration and the tangent modulus did not increase. Although damage of the construct was not shown by dose of A2P into culture medium as a consequence of its low reactivity, the tangent modulus did not statistically increase as collagen network had connected among chondrocytes.

Original languageEnglish
Title of host publication6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech
Pages899-902
Number of pages4
DOIs
Publication statusPublished - Oct 22 2010
Event6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech - Singapore, Singapore
Duration: Aug 1 2010Aug 6 2010

Publication series

NameIFMBE Proceedings
Volume31 IFMBE
ISSN (Print)1680-0737

Other

Other6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech
CountrySingapore
CitySingapore
Period8/1/108/6/10

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biomedical Engineering

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  • Cite this

    Omata, S., Shimizu, T., Sawae, Y., & Murakami, T. (2010). Effect of vitamin C on mechanical property of the regenerated-cartilage tissue. In 6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech (pp. 899-902). (IFMBE Proceedings; Vol. 31 IFMBE). https://doi.org/10.1007/978-3-642-14515-5_229