Effect of W62G mutation of hen lysozyme on the folding in vivo

Takatoshi Ohkuri, Taiji Imoto, Tadashi Ueda

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

In previous paper, we showed that W62G mutation caused ill effects at the early stages of folding of the reduced hen lysozyme in vitro. Here, we investigated whether the single mutation brings about drastic turn to in vivo folding of lysozyme. W62G lysozyme was secreted from yeast cells and then purified with ion-exchange chromatography. From the results of gel chromatography and peptide analysis, the species with two cysteines, Cys80 and Cys94, and non-native cystine, Cys64-Cys76, was partially present in secreted product of yeast containing gene for W62G lysozyme. Thus, it was suggested that W62G mutation also affected the in vivo folding of lysozyme.

Original languageEnglish
Pages (from-to)820-824
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume338
Issue number2
DOIs
Publication statusPublished - Dec 16 2005

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Muramidase
Mutation
Chromatography
Yeast
Yeasts
Cystine
Ion Exchange Chromatography
Gel Chromatography
Cysteine
Ion exchange
Genes
Gels
Cells
Peptides

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Effect of W62G mutation of hen lysozyme on the folding in vivo. / Ohkuri, Takatoshi; Imoto, Taiji; Ueda, Tadashi.

In: Biochemical and Biophysical Research Communications, Vol. 338, No. 2, 16.12.2005, p. 820-824.

Research output: Contribution to journalArticle

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