Effect of water activity control on the catalytic performance of surfactant - Arthromyces ramosus peroxidase complex in toluene

Noriho Kamiya, Teruyuki Nagamune

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Arthromyces ramosus peroxidase (ARP) was successfully modified with a synthetic surfactant for one-electron oxidation reaction of a hydrophobic substrate in toluene. Although UV-visible absorption spectrum of surfactant-ARP complex in toluene showed slight red shift of Soret band compared to that in water, the complex can catalyze oxidation reaction of o-phenylenediamine (o-PDA) with hydrogen peroxide. It appeared that thermodynamic water activity in the reaction system has dominant effect on either the catalytic activity or the stability in the catalytic cycle. Steady-state kinetics under the optimal condition revealed that the specific constant (kcat/Km) of ARP complex for o-PDA was 2 orders of magnitude lower than that in aqueous media, while only 13-fold lower for hydrogen peroxide. The reduction of catalytic activity caused by altering the reaction media from water to toluene was found to be mainly due to the low specific constant of ARP complex for o-PDA rather than hydrogen peroxide.

Original languageEnglish
Pages (from-to)55-59
Number of pages5
JournalBiochemical Engineering Journal
Issue number1
Publication statusPublished - Feb 14 2002
Externally publishedYes


All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Environmental Engineering
  • Bioengineering
  • Biomedical Engineering

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