Effective antisense DNA delivery by use of a polysaccharide/ polynucleotide complex

Ryouji Karinaga; Jusaku Minari; Kazuo Sakurai; Seiji Shinkai

doi:10.1295/koron.63.468

Effective antisense DNA delivery by use of a polysaccharide/ polynucleotide complex

Ryouji Karinaga, Jusaku Minari, Kazuo Sakurai, Seiji Shinkai

Institute for Advanced Study

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

A natural polysaccharides schizophyllan (SPG) can form a stoichiometric complex with polynucleotides. The complexed polynucleotide shows better anti-nuclease resistance than naked ones, indicating that the complex can be used as an olig-DNA carrier, especially to transport antisense DNA to intracellular mRNAs. In the present work, we attached β-cyclodextrin (βCyD) to SPG and a galactose moiety was connected to the βCyD by use of adamantine vs. βCyD interaction. The attached galactose increased the cellular ingestion when the complex was administeted to Hep G2, owing to the presence of asialoglycoprotein receptor on the cellular surface.

Original language	English
Pages (from-to)	468-475
Number of pages	8
Journal	KOBUNSHI RONBUNSHU
Volume	63
Issue number	7
DOIs	https://doi.org/10.1295/koron.63.468
Publication status	Published - Jul 1 2006

All Science Journal Classification (ASJC) codes

Chemical Engineering (miscellaneous)
Materials Science (miscellaneous)
Environmental Science(all)
Polymers and Plastics

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10.1295/koron.63.468

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abstract = "A natural polysaccharides schizophyllan (SPG) can form a stoichiometric complex with polynucleotides. The complexed polynucleotide shows better anti-nuclease resistance than naked ones, indicating that the complex can be used as an olig-DNA carrier, especially to transport antisense DNA to intracellular mRNAs. In the present work, we attached β-cyclodextrin (βCyD) to SPG and a galactose moiety was connected to the βCyD by use of adamantine vs. βCyD interaction. The attached galactose increased the cellular ingestion when the complex was administeted to Hep G2, owing to the presence of asialoglycoprotein receptor on the cellular surface.",

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AU - Karinaga, Ryouji

AU - Minari, Jusaku

AU - Sakurai, Kazuo

AU - Shinkai, Seiji

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