Abstract
A natural polysaccharides schizophyllan (SPG) can form a stoichiometric complex with polynucleotides. The complexed polynucleotide shows better anti-nuclease resistance than naked ones, indicating that the complex can be used as an olig-DNA carrier, especially to transport antisense DNA to intracellular mRNAs. In the present work, we attached β-cyclodextrin (βCyD) to SPG and a galactose moiety was connected to the βCyD by use of adamantine vs. βCyD interaction. The attached galactose increased the cellular ingestion when the complex was administeted to Hep G2, owing to the presence of asialoglycoprotein receptor on the cellular surface.
| Original language | English |
|---|---|
| Pages (from-to) | 468-475 |
| Number of pages | 8 |
| Journal | KOBUNSHI RONBUNSHU |
| Volume | 63 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - Jul 1 2006 |
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All Science Journal Classification (ASJC) codes
- Chemical Engineering (miscellaneous)
- Materials Science (miscellaneous)
- Environmental Science(all)
- Polymers and Plastics
Cite this
Effective antisense DNA delivery by use of a polysaccharide/ polynucleotide complex. / Karinaga, Ryouji; Minari, Jusaku; Sakurai, Kazuo; Shinkai, Seiji.
In: KOBUNSHI RONBUNSHU, Vol. 63, No. 7, 01.07.2006, p. 468-475.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effective antisense DNA delivery by use of a polysaccharide/ polynucleotide complex
AU - Karinaga, Ryouji
AU - Minari, Jusaku
AU - Sakurai, Kazuo
AU - Shinkai, Seiji
PY - 2006/7/1
Y1 - 2006/7/1
N2 - A natural polysaccharides schizophyllan (SPG) can form a stoichiometric complex with polynucleotides. The complexed polynucleotide shows better anti-nuclease resistance than naked ones, indicating that the complex can be used as an olig-DNA carrier, especially to transport antisense DNA to intracellular mRNAs. In the present work, we attached β-cyclodextrin (βCyD) to SPG and a galactose moiety was connected to the βCyD by use of adamantine vs. βCyD interaction. The attached galactose increased the cellular ingestion when the complex was administeted to Hep G2, owing to the presence of asialoglycoprotein receptor on the cellular surface.
AB - A natural polysaccharides schizophyllan (SPG) can form a stoichiometric complex with polynucleotides. The complexed polynucleotide shows better anti-nuclease resistance than naked ones, indicating that the complex can be used as an olig-DNA carrier, especially to transport antisense DNA to intracellular mRNAs. In the present work, we attached β-cyclodextrin (βCyD) to SPG and a galactose moiety was connected to the βCyD by use of adamantine vs. βCyD interaction. The attached galactose increased the cellular ingestion when the complex was administeted to Hep G2, owing to the presence of asialoglycoprotein receptor on the cellular surface.
UR - http://www.scopus.com/inward/record.url?scp=33748446203&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33748446203&partnerID=8YFLogxK
U2 - 10.1295/koron.63.468
DO - 10.1295/koron.63.468
M3 - Article
AN - SCOPUS:33748446203
VL - 63
SP - 468
EP - 475
JO - Kobunshi Ronbunshu
JF - Kobunshi Ronbunshu
SN - 0386-2186
IS - 7
ER -