Effective antisense DNA delivery by use of a polysaccharide/ polynucleotide complex

Ryouji Karinaga, Jusaku Minari, Kazuo Sakurai, Seiji Shinkai

Research output: Contribution to journalArticle

Abstract

A natural polysaccharides schizophyllan (SPG) can form a stoichiometric complex with polynucleotides. The complexed polynucleotide shows better anti-nuclease resistance than naked ones, indicating that the complex can be used as an olig-DNA carrier, especially to transport antisense DNA to intracellular mRNAs. In the present work, we attached β-cyclodextrin (βCyD) to SPG and a galactose moiety was connected to the βCyD by use of adamantine vs. βCyD interaction. The attached galactose increased the cellular ingestion when the complex was administeted to Hep G2, owing to the presence of asialoglycoprotein receptor on the cellular surface.

Original languageEnglish
Pages (from-to)468-475
Number of pages8
JournalKOBUNSHI RONBUNSHU
Volume63
Issue number7
DOIs
Publication statusPublished - Jul 1 2006

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Antisense DNA
Polynucleotides
Cyclodextrins
Polysaccharides
polysaccharide
DNA
Galactose
Sizofiran
Asialoglycoprotein Receptor
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Chemical Engineering (miscellaneous)
  • Materials Science (miscellaneous)
  • Environmental Science(all)
  • Polymers and Plastics

Cite this

Effective antisense DNA delivery by use of a polysaccharide/ polynucleotide complex. / Karinaga, Ryouji; Minari, Jusaku; Sakurai, Kazuo; Shinkai, Seiji.

In: KOBUNSHI RONBUNSHU, Vol. 63, No. 7, 01.07.2006, p. 468-475.

Research output: Contribution to journalArticle

Karinaga, Ryouji ; Minari, Jusaku ; Sakurai, Kazuo ; Shinkai, Seiji. / Effective antisense DNA delivery by use of a polysaccharide/ polynucleotide complex. In: KOBUNSHI RONBUNSHU. 2006 ; Vol. 63, No. 7. pp. 468-475.
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