Abstract
Electroporation is a useful means for non-viral gene delivery. Here, we investigated the use of electroporation to deliver polymer-DNA complexes into living cells using a protein kinase C (PKC)α-responsive polymer. The polymer was complexed with a luciferase-encoding DNA and electroporated into B16 melanoma cells. Gene expression from polymer-DNA complexes was 3- to 5-fold higher than from naked DNA. Moreover, after introduction of the polymer-DNA complex into tissues, luciferase levels were >2-fold higher in B16 melanoma tumors than in normal skin tissue. These results suggest that the combination of our polymer and electroporation is useful for the effective delivery of DNA into tumors.
| Original language | English |
|---|---|
| Pages (from-to) | 281-288 |
| Number of pages | 8 |
| Journal | Cancer Letters |
| Volume | 265 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Jul 8 2008 |
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All Science Journal Classification (ASJC) codes
- Oncology
- Cancer Research
Cite this
Effective delivery of DNA into tumor cells and tissues by electroporation of polymer-DNA complex. / Kang, Jeong Hun; Toita, Riki; Niidome, Takuro; Katayama, Yoshiki.
In: Cancer Letters, Vol. 265, No. 2, 08.07.2008, p. 281-288.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effective delivery of DNA into tumor cells and tissues by electroporation of polymer-DNA complex
AU - Kang, Jeong Hun
AU - Toita, Riki
AU - Niidome, Takuro
AU - Katayama, Yoshiki
PY - 2008/7/8
Y1 - 2008/7/8
N2 - Electroporation is a useful means for non-viral gene delivery. Here, we investigated the use of electroporation to deliver polymer-DNA complexes into living cells using a protein kinase C (PKC)α-responsive polymer. The polymer was complexed with a luciferase-encoding DNA and electroporated into B16 melanoma cells. Gene expression from polymer-DNA complexes was 3- to 5-fold higher than from naked DNA. Moreover, after introduction of the polymer-DNA complex into tissues, luciferase levels were >2-fold higher in B16 melanoma tumors than in normal skin tissue. These results suggest that the combination of our polymer and electroporation is useful for the effective delivery of DNA into tumors.
AB - Electroporation is a useful means for non-viral gene delivery. Here, we investigated the use of electroporation to deliver polymer-DNA complexes into living cells using a protein kinase C (PKC)α-responsive polymer. The polymer was complexed with a luciferase-encoding DNA and electroporated into B16 melanoma cells. Gene expression from polymer-DNA complexes was 3- to 5-fold higher than from naked DNA. Moreover, after introduction of the polymer-DNA complex into tissues, luciferase levels were >2-fold higher in B16 melanoma tumors than in normal skin tissue. These results suggest that the combination of our polymer and electroporation is useful for the effective delivery of DNA into tumors.
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UR - http://www.scopus.com/inward/citedby.url?scp=43949126845&partnerID=8YFLogxK
U2 - 10.1016/j.canlet.2008.02.045
DO - 10.1016/j.canlet.2008.02.045
M3 - Article
C2 - 18375054
AN - SCOPUS:43949126845
VL - 265
SP - 281
EP - 288
JO - Cancer Letters
JF - Cancer Letters
SN - 0304-3835
IS - 2
ER -