Effective delivery of DNA into tumor cells and tissues by electroporation of polymer-DNA complex

Jeong Hun Kang; Riki Toita; Takuro Niidome; Yoshiki Katayama

doi:10.1016/j.canlet.2008.02.045

Effective delivery of DNA into tumor cells and tissues by electroporation of polymer-DNA complex

Jeong Hun Kang, Riki Toita, Takuro Niidome, Yoshiki Katayama

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Electroporation is a useful means for non-viral gene delivery. Here, we investigated the use of electroporation to deliver polymer-DNA complexes into living cells using a protein kinase C (PKC)α-responsive polymer. The polymer was complexed with a luciferase-encoding DNA and electroporated into B16 melanoma cells. Gene expression from polymer-DNA complexes was 3- to 5-fold higher than from naked DNA. Moreover, after introduction of the polymer-DNA complex into tissues, luciferase levels were >2-fold higher in B16 melanoma tumors than in normal skin tissue. These results suggest that the combination of our polymer and electroporation is useful for the effective delivery of DNA into tumors.

Original language	English
Pages (from-to)	281-288
Number of pages	8
Journal	Cancer Letters
Volume	265
Issue number	2
DOIs	https://doi.org/10.1016/j.canlet.2008.02.045
Publication status	Published - Jul 8 2008

All Science Journal Classification (ASJC) codes

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.canlet.2008.02.045

Cite this

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title = "Effective delivery of DNA into tumor cells and tissues by electroporation of polymer-DNA complex",

abstract = "Electroporation is a useful means for non-viral gene delivery. Here, we investigated the use of electroporation to deliver polymer-DNA complexes into living cells using a protein kinase C (PKC)α-responsive polymer. The polymer was complexed with a luciferase-encoding DNA and electroporated into B16 melanoma cells. Gene expression from polymer-DNA complexes was 3- to 5-fold higher than from naked DNA. Moreover, after introduction of the polymer-DNA complex into tissues, luciferase levels were >2-fold higher in B16 melanoma tumors than in normal skin tissue. These results suggest that the combination of our polymer and electroporation is useful for the effective delivery of DNA into tumors.",

author = "Kang, {Jeong Hun} and Riki Toita and Takuro Niidome and Yoshiki Katayama",

note = "Funding Information: This work was supported by CREST, Japan Science and Technology Corporation, a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, Sports and Culture in Japan, and a Grant-in-Aid for Scientific Research from the Ministry of Health, Labour and Welfare.",

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doi = "10.1016/j.canlet.2008.02.045",

language = "English",

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journal = "Cancer Letters",

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T1 - Effective delivery of DNA into tumor cells and tissues by electroporation of polymer-DNA complex

AU - Kang, Jeong Hun

AU - Toita, Riki

AU - Niidome, Takuro

AU - Katayama, Yoshiki

N1 - Funding Information: This work was supported by CREST, Japan Science and Technology Corporation, a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, Sports and Culture in Japan, and a Grant-in-Aid for Scientific Research from the Ministry of Health, Labour and Welfare.

PY - 2008/7/8

Y1 - 2008/7/8

N2 - Electroporation is a useful means for non-viral gene delivery. Here, we investigated the use of electroporation to deliver polymer-DNA complexes into living cells using a protein kinase C (PKC)α-responsive polymer. The polymer was complexed with a luciferase-encoding DNA and electroporated into B16 melanoma cells. Gene expression from polymer-DNA complexes was 3- to 5-fold higher than from naked DNA. Moreover, after introduction of the polymer-DNA complex into tissues, luciferase levels were >2-fold higher in B16 melanoma tumors than in normal skin tissue. These results suggest that the combination of our polymer and electroporation is useful for the effective delivery of DNA into tumors.

AB - Electroporation is a useful means for non-viral gene delivery. Here, we investigated the use of electroporation to deliver polymer-DNA complexes into living cells using a protein kinase C (PKC)α-responsive polymer. The polymer was complexed with a luciferase-encoding DNA and electroporated into B16 melanoma cells. Gene expression from polymer-DNA complexes was 3- to 5-fold higher than from naked DNA. Moreover, after introduction of the polymer-DNA complex into tissues, luciferase levels were >2-fold higher in B16 melanoma tumors than in normal skin tissue. These results suggest that the combination of our polymer and electroporation is useful for the effective delivery of DNA into tumors.

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JO - Cancer Letters

JF - Cancer Letters

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Effective delivery of DNA into tumor cells and tissues by electroporation of polymer-DNA complex

Abstract

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