Effective delivery of DNA into tumor cells and tissues by electroporation of polymer-DNA complex

Jeong Hun Kang; Riki Toita; Takuro Niidome; Yoshiki Katayama

doi:10.1016/j.canlet.2008.02.045

Effective delivery of DNA into tumor cells and tissues by electroporation of polymer-DNA complex

Jeong Hun Kang, Riki Toita, Takuro Niidome, Yoshiki Katayama

Materials Science and Engineering, School of Engineering

Research output: Contribution to journal › Article

9 Citations (Scopus)

Abstract

Electroporation is a useful means for non-viral gene delivery. Here, we investigated the use of electroporation to deliver polymer-DNA complexes into living cells using a protein kinase C (PKC)α-responsive polymer. The polymer was complexed with a luciferase-encoding DNA and electroporated into B16 melanoma cells. Gene expression from polymer-DNA complexes was 3- to 5-fold higher than from naked DNA. Moreover, after introduction of the polymer-DNA complex into tissues, luciferase levels were >2-fold higher in B16 melanoma tumors than in normal skin tissue. These results suggest that the combination of our polymer and electroporation is useful for the effective delivery of DNA into tumors.

Original language	English
Pages (from-to)	281-288
Number of pages	8
Journal	Cancer Letters
Volume	265
Issue number	2
DOIs	https://doi.org/10.1016/j.canlet.2008.02.045
Publication status	Published - Jul 8 2008

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All Science Journal Classification (ASJC) codes

Oncology
Cancer Research

Cite this

Kang, J. H., Toita, R., Niidome, T., & Katayama, Y. (2008). Effective delivery of DNA into tumor cells and tissues by electroporation of polymer-DNA complex. Cancer Letters, 265(2), 281-288. https://doi.org/10.1016/j.canlet.2008.02.045

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10.1016/j.canlet.2008.02.045