Effective length calibration method for processing the fluorescence signal detected by charge-coupled device in capillary electrophoresis

Yi Ni, Chenchen Liu, Jin Chen, Qinmiao Chen, Xifang Zhu, Xiaoming Dou

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

A novel method named effective length calibration method has been developed to process the fluorescence signal detected by charge-coupled device during capillary electrophoresis. The new method treated each pixel as an individual point detector, and effectively binned a large number of pixels into a final electropherogram without losing the narrow detection window defined by a single pixel. Capillary electrophoresis separations of DNA were carried out and detected by charge-coupled device and conventional detector (photomultiplier tube). Detection properties including signal-to-noise ratio, peak width, detection frequency, and tilt of detector were investigated. It was found that the new method achieved much higher signal-to-noise ratio and smaller peak width than the conventional detector did. A Detection width of 0.5 μm was easily achieved.

Original languageEnglish
Pages (from-to)2054-2061
Number of pages8
JournalJournal of Separation Science
Volume40
Issue number9
DOIs
Publication statusPublished - May 2017
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Filtration and Separation

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