Effective method for discriminating between oral bacterial and human alkaline phosphatase activity

Yukie Shibata, Yoshihisa Yamashita, H. Miyazaki, S. Ueno, T. Takehara

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Alkaline phosphatase (ALPase) activity was quantitatively compared in various kinds of oral bacteria. High ALPase activity was detected in 3 species of periodontal bacteria, Porphyromonas gingivalis. Prevotella intermedia and Capno‐cvtophaga sputigena. The ALPase activity detected in these bacteria was almost completely inhibited in the presence of 1% sodium dodecyl sulfate (SDS). By contrast, the activity of mammalian ALPase isoenzymes was not inhibited at all even in the presence of 1% SDS. These results indicate that the ALPase assay in combination with 1% SDS can identify the origin of ALPase detected in gingival crevicular fluid as being from bacteria or from a host response. Clinical examination with adult periodontitis revealed that ALPase activity in gingival crevicular fluid from the patients consisted of a combination of SDS‐sensitive and SDS‐resistant activities. These findings indicate that ALPase activity detected in gingival crevicular fluid originates not only from bacteria but also from a host response.

Original languageEnglish
Pages (from-to)35-39
Number of pages5
JournalOral Microbiology and Immunology
Volume9
Issue number1
DOIs
Publication statusPublished - Jan 1 1994
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Dentistry(all)
  • Microbiology (medical)

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