Effective renaturation of met-1 lysozyme expressed in Escherichia Coli as inclusion bodies

Yoshitake Maeda, Tadashi Ueda, Taiji Imoto

Research output: Contribution to journalArticle

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Abstract

The effective renaturation method that we developed was applied to the renaturation of the reduced Met-1 lysozyme expressed in E. coli as inclusion bodies. Using a slow dialysis renaturation method, a concentration as high as 1.25 mg/ml of the reduced Met-1 lysozyme could be renatured in 40% yield, while the folding yield was less than 5% even at a concentration of 0.25 mg/ml using a rapid dilution method.

Original languageEnglish
Pages (from-to)67-74
Number of pages8
JournalProtein and Peptide Letters
Volume5
Issue number2
Publication statusPublished - Dec 1 1998

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Inclusion Bodies
Muramidase
Escherichia coli
Dialysis
Dilution

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biochemistry

Cite this

Effective renaturation of met-1 lysozyme expressed in Escherichia Coli as inclusion bodies. / Maeda, Yoshitake; Ueda, Tadashi; Imoto, Taiji.

In: Protein and Peptide Letters, Vol. 5, No. 2, 01.12.1998, p. 67-74.

Research output: Contribution to journalArticle

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