The effective renaturation method that we developed was applied to the renaturation of the reduced Met-1 lysozyme expressed in E. coli as inclusion bodies. Using a slow dialysis renaturation method, a concentration as high as 1.25 mg/ml of the reduced Met-1 lysozyme could be renatured in 40% yield, while the folding yield was less than 5% even at a concentration of 0.25 mg/ml using a rapid dilution method.
|Number of pages||8|
|Journal||Protein and Peptide Letters|
|Publication status||Published - Dec 1 1998|
All Science Journal Classification (ASJC) codes
- Structural Biology