Effective RNA interference in cultured silkworm cells mediated by overexpression of Caenorhabditis elegans SID-1

Hiroaki Mon, Isao Kobayashi, Shinji Ohkubo, Shuichiro Tomita, Man Lee, Hideki Sezutsu, Toshiki Tamura, Takahiro Kusakabe

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

RNA interference (RNAi) is a conserved mechanism that catalyzes sequence-specific gene silencing, and has been used for loss-of-function genetic screens in many organisms. Here, we demonstrated that the expression of Caenorhabditis elegans SID-1 (CeSID-1) could trigger effective gene silencing in the cultured silkworm cell line, BmN4 (BmN4-SID1). Soaking the BmN4-SID1 in dsRNA corresponding to endogenous target genes induced a significant decrease of the amount of mRNA or protein. A small amount of dsRNA was enough to silence the target gene in a few days. Overexpression of CeSID-1 did not affect the cell viability. Our results suggest that BmN4-SID1 can be used in many applications in silkworm cells and will become a valuable resource for gene analysis.

Original languageEnglish
Pages (from-to)40-46
Number of pages7
JournalRNA biology
Volume9
Issue number1
DOIs
Publication statusPublished - Jan 1 2012

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Bombyx
Sudden Infant Death
Caenorhabditis elegans
RNA Interference
Cultured Cells
Gene Silencing
Genes
Cell Survival
Cell Line
Messenger RNA
Proteins

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Effective RNA interference in cultured silkworm cells mediated by overexpression of Caenorhabditis elegans SID-1. / Mon, Hiroaki; Kobayashi, Isao; Ohkubo, Shinji; Tomita, Shuichiro; Lee, Man; Sezutsu, Hideki; Tamura, Toshiki; Kusakabe, Takahiro.

In: RNA biology, Vol. 9, No. 1, 01.01.2012, p. 40-46.

Research output: Contribution to journalArticle

Mon, Hiroaki ; Kobayashi, Isao ; Ohkubo, Shinji ; Tomita, Shuichiro ; Lee, Man ; Sezutsu, Hideki ; Tamura, Toshiki ; Kusakabe, Takahiro. / Effective RNA interference in cultured silkworm cells mediated by overexpression of Caenorhabditis elegans SID-1. In: RNA biology. 2012 ; Vol. 9, No. 1. pp. 40-46.
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