Effective RNA interference in cultured silkworm cells mediated by overexpression of Caenorhabditis elegans SID-1

Hiroaki Mon, Isao Kobayashi, Shinji Ohkubo, Shuichiro Tomita, Jae Man Lee, Hideki Sezutsu, Toshiki Tamura, Takahiro Kusakabe

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

RNA interference (RNAi) is a conserved mechanism that catalyzes sequence-specific gene silencing, and has been used for loss-of-function genetic screens in many organisms. Here, we demonstrated that the expression of Caenorhabditis elegans SID-1 (CeSID-1) could trigger effective gene silencing in the cultured silkworm cell line, BmN4 (BmN4-SID1). Soaking the BmN4-SID1 in dsRNA corresponding to endogenous target genes induced a significant decrease of the amount of mRNA or protein. A small amount of dsRNA was enough to silence the target gene in a few days. Overexpression of CeSID-1 did not affect the cell viability. Our results suggest that BmN4-SID1 can be used in many applications in silkworm cells and will become a valuable resource for gene analysis.

Original languageEnglish
Pages (from-to)40-46
Number of pages7
JournalRNA biology
Volume9
Issue number1
DOIs
Publication statusPublished - Jan 2012

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

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