Abstract
RNA interference (RNAi) is a conserved mechanism that catalyzes sequence-specific gene silencing, and has been used for loss-of-function genetic screens in many organisms. Here, we demonstrated that the expression of Caenorhabditis elegans SID-1 (CeSID-1) could trigger effective gene silencing in the cultured silkworm cell line, BmN4 (BmN4-SID1). Soaking the BmN4-SID1 in dsRNA corresponding to endogenous target genes induced a significant decrease of the amount of mRNA or protein. A small amount of dsRNA was enough to silence the target gene in a few days. Overexpression of CeSID-1 did not affect the cell viability. Our results suggest that BmN4-SID1 can be used in many applications in silkworm cells and will become a valuable resource for gene analysis.
| Original language | English |
|---|---|
| Pages (from-to) | 40-46 |
| Number of pages | 7 |
| Journal | RNA biology |
| Volume | 9 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 1 2012 |
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All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cell Biology
Cite this
Effective RNA interference in cultured silkworm cells mediated by overexpression of Caenorhabditis elegans SID-1. / Mon, Hiroaki; Kobayashi, Isao; Ohkubo, Shinji; Tomita, Shuichiro; Lee, Man; Sezutsu, Hideki; Tamura, Toshiki; Kusakabe, Takahiro.
In: RNA biology, Vol. 9, No. 1, 01.01.2012, p. 40-46.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effective RNA interference in cultured silkworm cells mediated by overexpression of Caenorhabditis elegans SID-1
AU - Mon, Hiroaki
AU - Kobayashi, Isao
AU - Ohkubo, Shinji
AU - Tomita, Shuichiro
AU - Lee, Man
AU - Sezutsu, Hideki
AU - Tamura, Toshiki
AU - Kusakabe, Takahiro
PY - 2012/1/1
Y1 - 2012/1/1
N2 - RNA interference (RNAi) is a conserved mechanism that catalyzes sequence-specific gene silencing, and has been used for loss-of-function genetic screens in many organisms. Here, we demonstrated that the expression of Caenorhabditis elegans SID-1 (CeSID-1) could trigger effective gene silencing in the cultured silkworm cell line, BmN4 (BmN4-SID1). Soaking the BmN4-SID1 in dsRNA corresponding to endogenous target genes induced a significant decrease of the amount of mRNA or protein. A small amount of dsRNA was enough to silence the target gene in a few days. Overexpression of CeSID-1 did not affect the cell viability. Our results suggest that BmN4-SID1 can be used in many applications in silkworm cells and will become a valuable resource for gene analysis.
AB - RNA interference (RNAi) is a conserved mechanism that catalyzes sequence-specific gene silencing, and has been used for loss-of-function genetic screens in many organisms. Here, we demonstrated that the expression of Caenorhabditis elegans SID-1 (CeSID-1) could trigger effective gene silencing in the cultured silkworm cell line, BmN4 (BmN4-SID1). Soaking the BmN4-SID1 in dsRNA corresponding to endogenous target genes induced a significant decrease of the amount of mRNA or protein. A small amount of dsRNA was enough to silence the target gene in a few days. Overexpression of CeSID-1 did not affect the cell viability. Our results suggest that BmN4-SID1 can be used in many applications in silkworm cells and will become a valuable resource for gene analysis.
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UR - http://www.scopus.com/inward/citedby.url?scp=84863011099&partnerID=8YFLogxK
U2 - 10.4161/rna.9.1.18084
DO - 10.4161/rna.9.1.18084
M3 - Article
VL - 9
SP - 40
EP - 46
JO - RNA Biology
JF - RNA Biology
SN - 1547-6286
IS - 1
ER -