Effective transgene expression without toxicity by intraperitoneal administration of PEG-detachable polyplex micelles in mice with peritoneal dissemination

Michiaki Kumagai, Shinya Shimoda, Rie Wakabayashi, Yumi Kunisawa, Takehiko Ishii, Kensuke Osada, Keiji Itaka, Nobuhiro Nishiyama, Kazunori Kataoka, kenji nakano

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Block copolymer of poly(ethylene glycol)-block-poly{N-[N-(2-aminoethyl)-2- aminoethyl]aspartamide} (PEG-P[Asp(DET)]) has been originally introduced as a promising gene carrier by forming a nanomicelle with plasmid DNA. In this study, the polyplex micelle of PEG-SS-P[Asp(DET)], which disulfide linkage (SS) between PEG and cationic polymer can detach the surrounding PEG chains upon intracellular reduction, was firstly evaluated with respect to in vivo transduction efficiency and toxicity in comparison to that of PEG-P[Asp(DET)] in peritoneally disseminated cancer model. Intraperitoneal (i.p.) administration of PEG-SS-P[Asp(DET)] polyplex micelles showed a higher (P < 0.05) transgene expression compared with PEG-P[Asp(DET)] in tumors. In contrast, the delivered distribution of the micelles was not different between the two polyplex micelles. PEG-SS-P[Asp(DET)] micelle encapsulating human tumor necrosis factor α (hTNF-α) gene exhibits a higher antitumor efficacy against disseminated cancer compared with PEG-P[Asp(DET)] or saline control. No hepatic and renal toxicities were observed by the administration of polyplex micelles. In conclusion, PEG-detachable polyplex micelles may represent an advantage in gene transduction in vivo over PEG-undetachable polyplex micelles after i.p. administration for peritoneal dissemination of cancer.

Original languageEnglish
Pages (from-to)542-551
Number of pages10
JournalJournal of Controlled Release
Volume160
Issue number3
DOIs
Publication statusPublished - Jun 28 2012

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DEET
Micelles
Transgenes
Neoplasms
Genes
polyethylene glycol-block-polyaspartic acid
Disulfides
Polymers
Plasmids
Kidney
polyethylene glycol bis(succinimidyl succinate)
Liver
DNA

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science

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Effective transgene expression without toxicity by intraperitoneal administration of PEG-detachable polyplex micelles in mice with peritoneal dissemination. / Kumagai, Michiaki; Shimoda, Shinya; Wakabayashi, Rie; Kunisawa, Yumi; Ishii, Takehiko; Osada, Kensuke; Itaka, Keiji; Nishiyama, Nobuhiro; Kataoka, Kazunori; nakano, kenji.

In: Journal of Controlled Release, Vol. 160, No. 3, 28.06.2012, p. 542-551.

Research output: Contribution to journalArticle

Kumagai, Michiaki ; Shimoda, Shinya ; Wakabayashi, Rie ; Kunisawa, Yumi ; Ishii, Takehiko ; Osada, Kensuke ; Itaka, Keiji ; Nishiyama, Nobuhiro ; Kataoka, Kazunori ; nakano, kenji. / Effective transgene expression without toxicity by intraperitoneal administration of PEG-detachable polyplex micelles in mice with peritoneal dissemination. In: Journal of Controlled Release. 2012 ; Vol. 160, No. 3. pp. 542-551.
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AU - Ishii, Takehiko

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