Effective tumor treatment by VEGF siRNA complexed with hydrophobic poly(amino acid)-modified polyethylenimine

Jie Chen, Huayu Tian, Xuan Dong, Zhaopei Guo, Zixue Jiao, Feifan Li, Arihiro Kano, Atsushi Maruyama, Xuesi Chen

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Two copolymers are designed and synthesised for siRNA delivery based on polyethylenimine by grafting hydrophilic acrylamide segments and hydrophobic poly(γ-benzyl L-glutamate). The amphiphilic PEI-PBLG/siRNA complex demonstrates high gene silencing efficiency in the absence or presence of 10vol% and 50vol% sera in vitro. The anti-tumor effects in vivo are evaluated in luciferase-bearing mice expressing CT26 tumors. PEI-PBLG/siVEGF complex provides a higher and more sustained suppressive effect by reducing VEGF mRNA expression in the tumors, leading to higher tumor growth inhibition efficacy. Further studies on the potential use of the PEI-PBLG carrier system in mediating the silencing of genes other than VEGF or in other tumor models are recommended. The amphiphilic copolymer conjugate PEI-PBLG can efficiently deliver siVEGF to tumor tissues and realize the downregulation of VEGF gene expression. The PEI-PBLG/siVEGF complex successfully exhibits an anti-angiogenic effect for the treatment of tumor growth inhibition in vivo.

Original languageEnglish
Pages (from-to)1438-1446
Number of pages9
JournalMacromolecular Bioscience
Volume13
Issue number10
DOIs
Publication statusPublished - Oct 1 2013

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Polyethyleneimine
Vascular Endothelial Growth Factor A
Small Interfering RNA
Polyetherimides
Amino acids
Tumors
Amino Acids
Neoplasms
Gene Silencing
Bearings (structural)
Copolymers
Genes
Acrylamide
Growth
Luciferases
Gene expression
Down-Regulation
polyethylenimine-poly(gamma-benzylglutamate)
Tissue
Gene Expression

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

Cite this

Effective tumor treatment by VEGF siRNA complexed with hydrophobic poly(amino acid)-modified polyethylenimine. / Chen, Jie; Tian, Huayu; Dong, Xuan; Guo, Zhaopei; Jiao, Zixue; Li, Feifan; Kano, Arihiro; Maruyama, Atsushi; Chen, Xuesi.

In: Macromolecular Bioscience, Vol. 13, No. 10, 01.10.2013, p. 1438-1446.

Research output: Contribution to journalArticle

Chen, Jie ; Tian, Huayu ; Dong, Xuan ; Guo, Zhaopei ; Jiao, Zixue ; Li, Feifan ; Kano, Arihiro ; Maruyama, Atsushi ; Chen, Xuesi. / Effective tumor treatment by VEGF siRNA complexed with hydrophobic poly(amino acid)-modified polyethylenimine. In: Macromolecular Bioscience. 2013 ; Vol. 13, No. 10. pp. 1438-1446.
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