Effective tumor treatment by VEGF siRNA complexed with hydrophobic poly(amino acid)-modified polyethylenimine

Jie Chen, Huayu Tian, Xuan Dong, Zhaopei Guo, Zixue Jiao, Feifan Li, Arihiro Kano, Atsushi Maruyama, Xuesi Chen

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Two copolymers are designed and synthesised for siRNA delivery based on polyethylenimine by grafting hydrophilic acrylamide segments and hydrophobic poly(γ-benzyl L-glutamate). The amphiphilic PEI-PBLG/siRNA complex demonstrates high gene silencing efficiency in the absence or presence of 10vol% and 50vol% sera in vitro. The anti-tumor effects in vivo are evaluated in luciferase-bearing mice expressing CT26 tumors. PEI-PBLG/siVEGF complex provides a higher and more sustained suppressive effect by reducing VEGF mRNA expression in the tumors, leading to higher tumor growth inhibition efficacy. Further studies on the potential use of the PEI-PBLG carrier system in mediating the silencing of genes other than VEGF or in other tumor models are recommended. The amphiphilic copolymer conjugate PEI-PBLG can efficiently deliver siVEGF to tumor tissues and realize the downregulation of VEGF gene expression. The PEI-PBLG/siVEGF complex successfully exhibits an anti-angiogenic effect for the treatment of tumor growth inhibition in vivo.

Original languageEnglish
Pages (from-to)1438-1446
Number of pages9
JournalMacromolecular Bioscience
Volume13
Issue number10
DOIs
Publication statusPublished - Oct 1 2013

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

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