Effectiveness of vitamin A acetate for enhancing the production of lung cancer specific monoclonal antibodies

Yuichi Inoue, Mihoko Fujisawa, Seiji Kawamoto, Masahiro Shoji, Shuichi Hashizume, Makoto Fujii, Yoshinori Katakura, Sanetaka Shirahata

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8 Citations (Scopus)

Abstract

The antibody productivity of the human-human hybridoma cell line AE6, which produces the tung cancer specific human monoclonal antibody AE6F4, was enhanced fourfold upon stimulation with 1 μg/ml of vitamin A acetate for one day. The enhancement lasted for about two weeks, and could be repeated by another stimulation with vitamin A acetate. The enhancing effect of vitamin A acetate was influenced by the cell density. Enhancement was clearly observed when the cell density was under 106 cells/ml. However, when the cell density was over 107 cells/ml, enhancement was observed weakly or not at all. Although the enhancing effect of vitamin A acetate is not unique to AE6 cells, not all human-human hybridoma cell lines show increased productivity upon VA acetate stimulation. This study suggests that the response to vitamin A acetate may be related to the properties of a particular fusion partner which the hybridoma cell inherits. The efficacy of vitamin A acetate for production of human monoclonal antibodies using human-human hybridomas is discussed.

Original languageEnglish
Pages (from-to)77-83
Number of pages7
JournalCytotechnology
Volume31
Issue number1-2
DOIs
Publication statusPublished - 1999

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Clinical Biochemistry
  • Cell Biology

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    Inoue, Y., Fujisawa, M., Kawamoto, S., Shoji, M., Hashizume, S., Fujii, M., Katakura, Y., & Shirahata, S. (1999). Effectiveness of vitamin A acetate for enhancing the production of lung cancer specific monoclonal antibodies. Cytotechnology, 31(1-2), 77-83. https://doi.org/10.1023/a:1008016020785