Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin on the development of murine palate in organ culture

Tomohiro Yamada, Kumiko Fujiwara, Katsuaki Mishima, Hideto Imura, Toshio Sugahara

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Objective: 2,3,7,8-Tetrachlorodibenzo-p-dioxin (tetrachlorodibenzodioxin) is known to cause cleft palates in pregnant mice in vivo. This study aimed at investigating the effects of tetrachlorodibenzodioxin on murine palatogenesis in vitro using organ culture. Materials and Methods: Palatal shelves from ICR mice foetuses (gestational day, 12.5) were removed and dissected to isolate the palatal tissue. Tissues were cultured in a chemically defined, serum-less medium using a suspension culture technique. Palatal fusion rates were assessed in suspension cultures containing tetrachlorodibenzodioxin at concentrations of 0.1, 1, 10 and 50 ng/mL and in non-treated controls. Palates from the control and 10 ng/mL groups were stained immunohistologically with antibodies against cleft palate-related factors (transforming growth factor-beta3, muscle segment homeobox 1, Lim-homeobox 8, fibroblast growth factor receptor 1, fibroblast growth factor receptor 2). Results: Over 90% of the palatal shelves completely fused in suspension culture after careful removal of the neural tissues. With tetrachlorodibenzodioxin addition, the palatal fusion rate decreased to 50% at a concentration of 1 ng/mL; however, the rate did not decrease any further at higher concentrations. Immunohistologically, transforming growth factor-beta3 disappeared earlier in the tetrachlorodibenzodioxin group. Conclusions: Tetrachlorodibenzodioxin contributes to the pathogenesis of cleft palate in vitro; however, direct intracellular effects are insufficient to fully account for the observed effects of tetrachlorodibenzodioxin, and it is possible that indirect mechanisms are also in play in vivo.

Original languageEnglish
Pages (from-to)185-189
Number of pages5
JournalAsian Journal of Oral and Maxillofacial Surgery
Volume19
Issue number4
DOIs
Publication statusPublished - Jan 1 2007

Fingerprint

Palate
Organ Culture Techniques
Transforming Growth Factor beta3
Cleft Palate
Suspensions
Homeobox Genes
Receptor, Fibroblast Growth Factor, Type 2
Receptor, Fibroblast Growth Factor, Type 1
Culture Techniques
Inbred ICR Mouse
Polychlorinated Dibenzodioxins
Fetus
Muscles
Antibodies
Serum

All Science Journal Classification (ASJC) codes

  • Surgery
  • Oral Surgery
  • Orthopedics and Sports Medicine

Cite this

Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin on the development of murine palate in organ culture. / Yamada, Tomohiro; Fujiwara, Kumiko; Mishima, Katsuaki; Imura, Hideto; Sugahara, Toshio.

In: Asian Journal of Oral and Maxillofacial Surgery, Vol. 19, No. 4, 01.01.2007, p. 185-189.

Research output: Contribution to journalArticle

Yamada, Tomohiro ; Fujiwara, Kumiko ; Mishima, Katsuaki ; Imura, Hideto ; Sugahara, Toshio. / Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin on the development of murine palate in organ culture. In: Asian Journal of Oral and Maxillofacial Surgery. 2007 ; Vol. 19, No. 4. pp. 185-189.
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abstract = "Objective: 2,3,7,8-Tetrachlorodibenzo-p-dioxin (tetrachlorodibenzodioxin) is known to cause cleft palates in pregnant mice in vivo. This study aimed at investigating the effects of tetrachlorodibenzodioxin on murine palatogenesis in vitro using organ culture. Materials and Methods: Palatal shelves from ICR mice foetuses (gestational day, 12.5) were removed and dissected to isolate the palatal tissue. Tissues were cultured in a chemically defined, serum-less medium using a suspension culture technique. Palatal fusion rates were assessed in suspension cultures containing tetrachlorodibenzodioxin at concentrations of 0.1, 1, 10 and 50 ng/mL and in non-treated controls. Palates from the control and 10 ng/mL groups were stained immunohistologically with antibodies against cleft palate-related factors (transforming growth factor-beta3, muscle segment homeobox 1, Lim-homeobox 8, fibroblast growth factor receptor 1, fibroblast growth factor receptor 2). Results: Over 90{\%} of the palatal shelves completely fused in suspension culture after careful removal of the neural tissues. With tetrachlorodibenzodioxin addition, the palatal fusion rate decreased to 50{\%} at a concentration of 1 ng/mL; however, the rate did not decrease any further at higher concentrations. Immunohistologically, transforming growth factor-beta3 disappeared earlier in the tetrachlorodibenzodioxin group. Conclusions: Tetrachlorodibenzodioxin contributes to the pathogenesis of cleft palate in vitro; however, direct intracellular effects are insufficient to fully account for the observed effects of tetrachlorodibenzodioxin, and it is possible that indirect mechanisms are also in play in vivo.",
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T1 - Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin on the development of murine palate in organ culture

AU - Yamada, Tomohiro

AU - Fujiwara, Kumiko

AU - Mishima, Katsuaki

AU - Imura, Hideto

AU - Sugahara, Toshio

PY - 2007/1/1

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N2 - Objective: 2,3,7,8-Tetrachlorodibenzo-p-dioxin (tetrachlorodibenzodioxin) is known to cause cleft palates in pregnant mice in vivo. This study aimed at investigating the effects of tetrachlorodibenzodioxin on murine palatogenesis in vitro using organ culture. Materials and Methods: Palatal shelves from ICR mice foetuses (gestational day, 12.5) were removed and dissected to isolate the palatal tissue. Tissues were cultured in a chemically defined, serum-less medium using a suspension culture technique. Palatal fusion rates were assessed in suspension cultures containing tetrachlorodibenzodioxin at concentrations of 0.1, 1, 10 and 50 ng/mL and in non-treated controls. Palates from the control and 10 ng/mL groups were stained immunohistologically with antibodies against cleft palate-related factors (transforming growth factor-beta3, muscle segment homeobox 1, Lim-homeobox 8, fibroblast growth factor receptor 1, fibroblast growth factor receptor 2). Results: Over 90% of the palatal shelves completely fused in suspension culture after careful removal of the neural tissues. With tetrachlorodibenzodioxin addition, the palatal fusion rate decreased to 50% at a concentration of 1 ng/mL; however, the rate did not decrease any further at higher concentrations. Immunohistologically, transforming growth factor-beta3 disappeared earlier in the tetrachlorodibenzodioxin group. Conclusions: Tetrachlorodibenzodioxin contributes to the pathogenesis of cleft palate in vitro; however, direct intracellular effects are insufficient to fully account for the observed effects of tetrachlorodibenzodioxin, and it is possible that indirect mechanisms are also in play in vivo.

AB - Objective: 2,3,7,8-Tetrachlorodibenzo-p-dioxin (tetrachlorodibenzodioxin) is known to cause cleft palates in pregnant mice in vivo. This study aimed at investigating the effects of tetrachlorodibenzodioxin on murine palatogenesis in vitro using organ culture. Materials and Methods: Palatal shelves from ICR mice foetuses (gestational day, 12.5) were removed and dissected to isolate the palatal tissue. Tissues were cultured in a chemically defined, serum-less medium using a suspension culture technique. Palatal fusion rates were assessed in suspension cultures containing tetrachlorodibenzodioxin at concentrations of 0.1, 1, 10 and 50 ng/mL and in non-treated controls. Palates from the control and 10 ng/mL groups were stained immunohistologically with antibodies against cleft palate-related factors (transforming growth factor-beta3, muscle segment homeobox 1, Lim-homeobox 8, fibroblast growth factor receptor 1, fibroblast growth factor receptor 2). Results: Over 90% of the palatal shelves completely fused in suspension culture after careful removal of the neural tissues. With tetrachlorodibenzodioxin addition, the palatal fusion rate decreased to 50% at a concentration of 1 ng/mL; however, the rate did not decrease any further at higher concentrations. Immunohistologically, transforming growth factor-beta3 disappeared earlier in the tetrachlorodibenzodioxin group. Conclusions: Tetrachlorodibenzodioxin contributes to the pathogenesis of cleft palate in vitro; however, direct intracellular effects are insufficient to fully account for the observed effects of tetrachlorodibenzodioxin, and it is possible that indirect mechanisms are also in play in vivo.

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