Effects of 3-methylsulphonyl-4,5,3′,4′-tetrachlorobiphenyl and 7,8-benzoflavone on mouse liver aryl hydrocarbon hydroxylase activity in vitro

Chikako Kiyohara, T. Hirohata, N. Mohri, Y. Masuda

Research output: Contribution to journalArticle

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Abstract

The activity of aryl hydrocarbon hydroxylase (AHH) in mouse liver microsomes was assayed in the presence of 3-methylsulphonyl-4,5,3′,4′-tetrachlorobiphenyl (3-MSF-TCB). The mice had been previously injected with a fixed amount of the AHH inducer 3-methylcholanthrene (MC) or 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) or with the vehicle (olive oil) alone. The dose-effect patterns for 3-MSF-TCB were markedly different according to the genetic responsiveness of the mice towards aromatic hydrocarbons (Ah responsiveness); in Ah responsive strains 3-MSF-TCB inhibited the MC-induced AHH activity as did 7,8-benzoflavone (ANF), which is known to be a potent inhibitor of AHH, whereas in Ah non-responsive strains 3-MSF-TCB (and ANF) greatly enhanced the same activity. On the other hand, the dose-response patterns were similar for both types of mice for TCDD-induced activity or basal activity (mice injected with the vehicle alone). The dose-effect curves for 3-MSF-TCB were quite distinct from those for ANF, particularly with respect to the basal AHH activity, for both Ah responsive and non-responsive strains. These results indicate that both ANF and 3-MSF-TCB can have either an inhibitory or an activating effect on AHH, depending on the dose, the Ah phenotype and previous induction with MC or TCDD.

Original languageEnglish
Pages (from-to)103-107
Number of pages5
JournalToxicology in Vitro
Volume4
Issue number2
DOIs
Publication statusPublished - Jan 1 1990

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Aryl Hydrocarbon Hydroxylases
Liver
Atrial Natriuretic Factor
Methylcholanthrene
Aromatic Hydrocarbons
Liver Microsomes
In Vitro Techniques
alpha-naphthoflavone
3-methylsulfonyl-4,5,3',4'-tetrachlorobiphenyl
Phenotype
Polychlorinated Dibenzodioxins
1,4-dioxin

All Science Journal Classification (ASJC) codes

  • Toxicology

Cite this

Effects of 3-methylsulphonyl-4,5,3′,4′-tetrachlorobiphenyl and 7,8-benzoflavone on mouse liver aryl hydrocarbon hydroxylase activity in vitro. / Kiyohara, Chikako; Hirohata, T.; Mohri, N.; Masuda, Y.

In: Toxicology in Vitro, Vol. 4, No. 2, 01.01.1990, p. 103-107.

Research output: Contribution to journalArticle

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