Effects of apatite cement containing atelocollagen on attachment to and proliferation and differentiation of MC3T3-E1 osteoblastic cells

Masaaki Takechi, Yoshiaki Ninomiya, Kouji Ohta, Misato Tada, Kazuki Sasaki, Mohammad Zeshaan Rahman, Akira Ohta, Kanji Tsuru, Kunio Ishikawa

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

To improve the osteoconductivity of apatite cement (AC) for reconstruction of bone defects after oral maxillofacial surgery, we previously fabricated AC containing atelocollagen (AC(ate)). In the present study, we examined the initial attachment, proliferation and differentiation of mouse osteoblastic cells (MC3T3-E1 cells) on the surface of conventional AC (c-AC), AC(ate) and a plastic cell dish. The number of osteoblastic cells showing initial attachment to AC(ate) was greater than those attached to c-AC and similar to the number attached to the plastic cell wells. We also found that osteoblastic cells were well spread and increased their number on AC(ate) in comparison with c-AC and the wells without specimens, while the amount of procollagen type I carboxy-terminal peptide (PIPC) produced in osteoblastic cells after three days on AC(ate) was greater as compared to the others. There was no significant difference in regard to alkaline phosphatase (ALP) activity and osteocalcin production by osteoblastic cells among the three surface types after three and six days. However, after 12 days, ALP activity and the produced osteocalcin were greater with AC(ate). In conclusion, AC(ate) may be a useful material with high osteoconductivity for reconstruction of bone defects after oral maxillofacial surgery.

Original languageEnglish
Article number283
JournalMaterials
Volume9
Issue number4
DOIs
Publication statusPublished - Jan 1 2016

Fingerprint

Apatites
Apatite
Cements
Osteocalcin
Phosphatases
Surgery
Alkaline Phosphatase
Bone
Plastics
atelocollagen
Bone cement
Bone Cements
Defects
Collagen Type I
Peptides

All Science Journal Classification (ASJC) codes

  • Materials Science(all)

Cite this

Effects of apatite cement containing atelocollagen on attachment to and proliferation and differentiation of MC3T3-E1 osteoblastic cells. / Takechi, Masaaki; Ninomiya, Yoshiaki; Ohta, Kouji; Tada, Misato; Sasaki, Kazuki; Rahman, Mohammad Zeshaan; Ohta, Akira; Tsuru, Kanji; Ishikawa, Kunio.

In: Materials, Vol. 9, No. 4, 283, 01.01.2016.

Research output: Contribution to journalArticle

Takechi, Masaaki ; Ninomiya, Yoshiaki ; Ohta, Kouji ; Tada, Misato ; Sasaki, Kazuki ; Rahman, Mohammad Zeshaan ; Ohta, Akira ; Tsuru, Kanji ; Ishikawa, Kunio. / Effects of apatite cement containing atelocollagen on attachment to and proliferation and differentiation of MC3T3-E1 osteoblastic cells. In: Materials. 2016 ; Vol. 9, No. 4.
@article{d37769052a164a939827c18e0c6901da,
title = "Effects of apatite cement containing atelocollagen on attachment to and proliferation and differentiation of MC3T3-E1 osteoblastic cells",
abstract = "To improve the osteoconductivity of apatite cement (AC) for reconstruction of bone defects after oral maxillofacial surgery, we previously fabricated AC containing atelocollagen (AC(ate)). In the present study, we examined the initial attachment, proliferation and differentiation of mouse osteoblastic cells (MC3T3-E1 cells) on the surface of conventional AC (c-AC), AC(ate) and a plastic cell dish. The number of osteoblastic cells showing initial attachment to AC(ate) was greater than those attached to c-AC and similar to the number attached to the plastic cell wells. We also found that osteoblastic cells were well spread and increased their number on AC(ate) in comparison with c-AC and the wells without specimens, while the amount of procollagen type I carboxy-terminal peptide (PIPC) produced in osteoblastic cells after three days on AC(ate) was greater as compared to the others. There was no significant difference in regard to alkaline phosphatase (ALP) activity and osteocalcin production by osteoblastic cells among the three surface types after three and six days. However, after 12 days, ALP activity and the produced osteocalcin were greater with AC(ate). In conclusion, AC(ate) may be a useful material with high osteoconductivity for reconstruction of bone defects after oral maxillofacial surgery.",
author = "Masaaki Takechi and Yoshiaki Ninomiya and Kouji Ohta and Misato Tada and Kazuki Sasaki and Rahman, {Mohammad Zeshaan} and Akira Ohta and Kanji Tsuru and Kunio Ishikawa",
year = "2016",
month = "1",
day = "1",
doi = "10.3390/ma9040283",
language = "English",
volume = "9",
journal = "Materials",
issn = "1996-1944",
publisher = "MDPI AG",
number = "4",

}

TY - JOUR

T1 - Effects of apatite cement containing atelocollagen on attachment to and proliferation and differentiation of MC3T3-E1 osteoblastic cells

AU - Takechi, Masaaki

AU - Ninomiya, Yoshiaki

AU - Ohta, Kouji

AU - Tada, Misato

AU - Sasaki, Kazuki

AU - Rahman, Mohammad Zeshaan

AU - Ohta, Akira

AU - Tsuru, Kanji

AU - Ishikawa, Kunio

PY - 2016/1/1

Y1 - 2016/1/1

N2 - To improve the osteoconductivity of apatite cement (AC) for reconstruction of bone defects after oral maxillofacial surgery, we previously fabricated AC containing atelocollagen (AC(ate)). In the present study, we examined the initial attachment, proliferation and differentiation of mouse osteoblastic cells (MC3T3-E1 cells) on the surface of conventional AC (c-AC), AC(ate) and a plastic cell dish. The number of osteoblastic cells showing initial attachment to AC(ate) was greater than those attached to c-AC and similar to the number attached to the plastic cell wells. We also found that osteoblastic cells were well spread and increased their number on AC(ate) in comparison with c-AC and the wells without specimens, while the amount of procollagen type I carboxy-terminal peptide (PIPC) produced in osteoblastic cells after three days on AC(ate) was greater as compared to the others. There was no significant difference in regard to alkaline phosphatase (ALP) activity and osteocalcin production by osteoblastic cells among the three surface types after three and six days. However, after 12 days, ALP activity and the produced osteocalcin were greater with AC(ate). In conclusion, AC(ate) may be a useful material with high osteoconductivity for reconstruction of bone defects after oral maxillofacial surgery.

AB - To improve the osteoconductivity of apatite cement (AC) for reconstruction of bone defects after oral maxillofacial surgery, we previously fabricated AC containing atelocollagen (AC(ate)). In the present study, we examined the initial attachment, proliferation and differentiation of mouse osteoblastic cells (MC3T3-E1 cells) on the surface of conventional AC (c-AC), AC(ate) and a plastic cell dish. The number of osteoblastic cells showing initial attachment to AC(ate) was greater than those attached to c-AC and similar to the number attached to the plastic cell wells. We also found that osteoblastic cells were well spread and increased their number on AC(ate) in comparison with c-AC and the wells without specimens, while the amount of procollagen type I carboxy-terminal peptide (PIPC) produced in osteoblastic cells after three days on AC(ate) was greater as compared to the others. There was no significant difference in regard to alkaline phosphatase (ALP) activity and osteocalcin production by osteoblastic cells among the three surface types after three and six days. However, after 12 days, ALP activity and the produced osteocalcin were greater with AC(ate). In conclusion, AC(ate) may be a useful material with high osteoconductivity for reconstruction of bone defects after oral maxillofacial surgery.

UR - http://www.scopus.com/inward/record.url?scp=84965010242&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84965010242&partnerID=8YFLogxK

U2 - 10.3390/ma9040283

DO - 10.3390/ma9040283

M3 - Article

AN - SCOPUS:84965010242

VL - 9

JO - Materials

JF - Materials

SN - 1996-1944

IS - 4

M1 - 283

ER -