Effects of bacteriocins on methicillin-resistant Staphylococcus aureus biofilm

Ken Ichi Okuda, Takeshi Zendo, Shinya Sugimoto, Tadayuki Iwase, Akiko Tajima, Satomi Yamada, Kenji Sonomoto, Yoshimitsu Mizunoe

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

Control of biofilms formed by microbial pathogens is an important subject for medical researchers, since the development of biofilms on foreign-body surfaces often causes biofilm-associated infections in patients with indwelling medical devices. The present study examined the effects of different kinds of bacteriocins, which are ribosomally synthesized antimicrobial peptides produced by certain bacteria, on biofilms formed by a clinical isolate of methicillin-resistant Staphylococcus aureus (MRSA). The activities and modes of action of three bacteriocins with different structures (nisin A, lacticin Q, and nukacin ISK-1) were evaluated. Vancomycin, a glycopeptide antibiotic used in the treatment of MRSA infections, showed bactericidal activity against planktonic cells but not against biofilm cells. Among the tested bacteriocins, nisin A showed the highest bactericidal activity against both planktonic cells and biofilm cells. Lacticin Q also showed bactericidal activity against both planktonic cells and biofilm cells, but its activity against biofilm cells was significantly lower than that of nisin A. Nukacin ISK-1 showed bacteriostatic activity against planktonic cells and did not show bactericidal activity against biofilm cells. Mode-of-action studies indicated that pore formation leading to ATP efflux is important for the bactericidal activity against biofilm cells. Our results suggest that bacteriocins that form stable pores on biofilm cells are highly potent for the treatment of MRSA biofilm infections.

Original languageEnglish
Pages (from-to)5572-5579
Number of pages8
JournalAntimicrobial Agents and Chemotherapy
Volume57
Issue number11
DOIs
Publication statusPublished - Nov 1 2013

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Bacteriocins
Methicillin-Resistant Staphylococcus aureus
Biofilms
Infection
Glycopeptides
Vancomycin
Foreign Bodies
Adenosine Triphosphate

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

Cite this

Okuda, K. I., Zendo, T., Sugimoto, S., Iwase, T., Tajima, A., Yamada, S., ... Mizunoe, Y. (2013). Effects of bacteriocins on methicillin-resistant Staphylococcus aureus biofilm. Antimicrobial Agents and Chemotherapy, 57(11), 5572-5579. https://doi.org/10.1128/AAC.00888-13

Effects of bacteriocins on methicillin-resistant Staphylococcus aureus biofilm. / Okuda, Ken Ichi; Zendo, Takeshi; Sugimoto, Shinya; Iwase, Tadayuki; Tajima, Akiko; Yamada, Satomi; Sonomoto, Kenji; Mizunoe, Yoshimitsu.

In: Antimicrobial Agents and Chemotherapy, Vol. 57, No. 11, 01.11.2013, p. 5572-5579.

Research output: Contribution to journalArticle

Okuda, KI, Zendo, T, Sugimoto, S, Iwase, T, Tajima, A, Yamada, S, Sonomoto, K & Mizunoe, Y 2013, 'Effects of bacteriocins on methicillin-resistant Staphylococcus aureus biofilm', Antimicrobial Agents and Chemotherapy, vol. 57, no. 11, pp. 5572-5579. https://doi.org/10.1128/AAC.00888-13
Okuda, Ken Ichi ; Zendo, Takeshi ; Sugimoto, Shinya ; Iwase, Tadayuki ; Tajima, Akiko ; Yamada, Satomi ; Sonomoto, Kenji ; Mizunoe, Yoshimitsu. / Effects of bacteriocins on methicillin-resistant Staphylococcus aureus biofilm. In: Antimicrobial Agents and Chemotherapy. 2013 ; Vol. 57, No. 11. pp. 5572-5579.
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