Abstract
Improvement of oral epithelial adhesion to titanium (Ti) may significantly enhance the efficacy of dental implants. We aimed to investigate whether calcium chloride (CaCl2) hydrothermally treated (HT) Ti could promote sealing of the peri-implant epithelium (PIE) around the implant. Right maxillary first molars were extracted from rats and replaced with either CaCl2-HT implants (Ca-HT group), distilled water-HT implants (DW-HT group), or untreated implants (Cont group). After 4 weeks, the implant-PIE interface of the Ca-HT group exhibited a band of immunoreactive laminin-332, similar to the tooth-junctional epithelium interface, which was absent in the Cont and DW-HT groups at the upper portion. We also investigated the effect of Ca-HT on the attachment of rat oral epithelial cells (OECs). OEC adherence onto Ca-HT Ti plates was stronger with higher expression levels of adhesion proteins compared with Cont and DW-HT groups. These results indicate that HT with CaCl2 improves the integration of soft tissue cells with the Ti implant at 4 weeks after implantation, which might facilitate the development of a soft tissue barrier around the implant.
| Original language | English |
|---|---|
| Pages (from-to) | 141-147 |
| Number of pages | 7 |
| Journal | Colloids and Surfaces B: Biointerfaces |
| Volume | 131 |
| DOIs | |
| Publication status | Published - Jul 1 2015 |
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All Science Journal Classification (ASJC) codes
- Biotechnology
- Surfaces and Interfaces
- Physical and Theoretical Chemistry
- Colloid and Surface Chemistry
Cite this
Effects of CaCl2 hydrothermal treatment of titanium implant surfaces on early epithelial sealing. / Oshiro, Wakana; Ayukawa, Yasunori; Ikiru, Atsuta; Furuhashi, Akihiro; Yamazoe, Junichi; Kondo, Ryosuke; Sakaguchi, Mami; Matsuura, Yuri; Tsukiyama, Yoshihiro; Koyano, Kiyoshi.
In: Colloids and Surfaces B: Biointerfaces, Vol. 131, 01.07.2015, p. 141-147.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effects of CaCl2 hydrothermal treatment of titanium implant surfaces on early epithelial sealing
AU - Oshiro, Wakana
AU - Ayukawa, Yasunori
AU - Ikiru, Atsuta
AU - Furuhashi, Akihiro
AU - Yamazoe, Junichi
AU - Kondo, Ryosuke
AU - Sakaguchi, Mami
AU - Matsuura, Yuri
AU - Tsukiyama, Yoshihiro
AU - Koyano, Kiyoshi
PY - 2015/7/1
Y1 - 2015/7/1
N2 - Improvement of oral epithelial adhesion to titanium (Ti) may significantly enhance the efficacy of dental implants. We aimed to investigate whether calcium chloride (CaCl2) hydrothermally treated (HT) Ti could promote sealing of the peri-implant epithelium (PIE) around the implant. Right maxillary first molars were extracted from rats and replaced with either CaCl2-HT implants (Ca-HT group), distilled water-HT implants (DW-HT group), or untreated implants (Cont group). After 4 weeks, the implant-PIE interface of the Ca-HT group exhibited a band of immunoreactive laminin-332, similar to the tooth-junctional epithelium interface, which was absent in the Cont and DW-HT groups at the upper portion. We also investigated the effect of Ca-HT on the attachment of rat oral epithelial cells (OECs). OEC adherence onto Ca-HT Ti plates was stronger with higher expression levels of adhesion proteins compared with Cont and DW-HT groups. These results indicate that HT with CaCl2 improves the integration of soft tissue cells with the Ti implant at 4 weeks after implantation, which might facilitate the development of a soft tissue barrier around the implant.
AB - Improvement of oral epithelial adhesion to titanium (Ti) may significantly enhance the efficacy of dental implants. We aimed to investigate whether calcium chloride (CaCl2) hydrothermally treated (HT) Ti could promote sealing of the peri-implant epithelium (PIE) around the implant. Right maxillary first molars were extracted from rats and replaced with either CaCl2-HT implants (Ca-HT group), distilled water-HT implants (DW-HT group), or untreated implants (Cont group). After 4 weeks, the implant-PIE interface of the Ca-HT group exhibited a band of immunoreactive laminin-332, similar to the tooth-junctional epithelium interface, which was absent in the Cont and DW-HT groups at the upper portion. We also investigated the effect of Ca-HT on the attachment of rat oral epithelial cells (OECs). OEC adherence onto Ca-HT Ti plates was stronger with higher expression levels of adhesion proteins compared with Cont and DW-HT groups. These results indicate that HT with CaCl2 improves the integration of soft tissue cells with the Ti implant at 4 weeks after implantation, which might facilitate the development of a soft tissue barrier around the implant.
UR - http://www.scopus.com/inward/record.url?scp=84936938401&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84936938401&partnerID=8YFLogxK
U2 - 10.1016/j.colsurfb.2015.04.025
DO - 10.1016/j.colsurfb.2015.04.025
M3 - Article
C2 - 25982317
AN - SCOPUS:84936938401
VL - 131
SP - 141
EP - 147
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
SN - 0927-7765
ER -