Effects of cAMP- and cGMP-dependent protein kinases, and calmodulin on Ca 2+ uptake by highly purified sarcolemmal vesicles of vascular smooth muscle

Eiichi Suematsu, Masato Hirata, Hirosi Kuriyama

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

Sarcolemmal fractions of vascular smooth muscles were prepared from porcine thoracic aortae by differential and sucrose density gradient centrifugation. In these fractions, there was a high activity of 5′-nucleotidase, a putative marker enzyme of plasma membrane, and a low activity of rotenone insensitive NADH-cytochrome c reductase a marker of sarcoplasmic reticulum. In these fractions, the Ca 2+ uptake was ATP-dependent. A low concentration of saponin which inhibited Ca 2+ uptake by the plasma membrane but not by the sarcoplasmic reticulum, inhibited 65% of the Ca 2+ uptake of this fraction. The Ca 2+ uptake of this fraction was enhanced by cAMP- and cGMP-dependent protein kinases, and by calmodulin. The cAMP-dependent protein kinase enhanced the phosphorylation of 28 and 22 kDA proteins, while the cGMP-dependent protein kinase phosphorylated the 35 kDa protein. The phosphorylation of 100, 75, 65, 41 and 22 kDa proteins was enhanced by Ca 2+ and calmodulin. These results indicate that cAMP- and cGMP-dependent protein kinases as well as calmodulin play important roles in Ca 2+ transport in the sarcolemma, and that the phosphorylated proteins may be associated with an enhancement of Ca 2+ transport in the sarcolemma.

Original languageEnglish
Pages (from-to)83-90
Number of pages8
JournalBBA - Biomembranes
Volume773
Issue number1
DOIs
Publication statusPublished - Jun 13 1984

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Cyclic GMP-Dependent Protein Kinases
Calmodulin
Cyclic AMP-Dependent Protein Kinases
Vascular Smooth Muscle
Muscle
Sarcolemma
Phosphorylation
Sarcoplasmic Reticulum
Cell membranes
Proteins
Cell Membrane
NADH Dehydrogenase
Rotenone
5'-Nucleotidase
Density Gradient Centrifugation
Centrifugation
Saponins
Thoracic Aorta
Sucrose
Swine

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Cell Biology

Cite this

Effects of cAMP- and cGMP-dependent protein kinases, and calmodulin on Ca 2+ uptake by highly purified sarcolemmal vesicles of vascular smooth muscle . / Suematsu, Eiichi; Hirata, Masato; Kuriyama, Hirosi.

In: BBA - Biomembranes, Vol. 773, No. 1, 13.06.1984, p. 83-90.

Research output: Contribution to journalArticle

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