TY - JOUR
T1 - Effects of CeO2 nanoparticles on microbial metabolism
AU - Masaki, Shota
AU - Shiotsu, Hiroyuki
AU - Ohnuki, Toshihiko
AU - Sakamoto, Fuminori
AU - Utsunomiya, Satoshi
N1 - Funding Information:
The authors would like to thank the members of the bio-actinide group at JAEA for providing technical support for HPLC and electrophoretic analyses and the staff of the research support center, Department of Agronomy, Kyushu University for providing technical support for LCMS-IT-TOF. The authors are also grateful to Professor Fein for his helpful advice on the revision. This work was partially supported through funding from Grants-in-Aid for Scientific Research through the Ministry of Education, Culture, Sports, Science and Technology in Japan and the Basic Research Fund of the Radioactive Waste Management Funding and Research Center .
Publisher Copyright:
© 2014 Elsevier B.V.
PY - 2015/1/6
Y1 - 2015/1/6
N2 - To understand the effects of nanoparticles on microorganisms, we experimentally investigated the effects of CeO2 nanoparticles (CeNPs) on yeast (S. cerevisiae) focusing on microbial metabolites and intracellular proteins. The yeast were harvested from a yeast extract peptone dextrose medium containing 0, 10, 100, and 250ppm of CeNPs and incubated for 120h in 1mM NaCl solution at three different pH values: 3, 5, and 7. The yeast released organic matter, P, K, and Mg into the NaCl solution at all pH values, even without CeNPs. Distinct differences were detected in the released organic species and intracellular proteins after exposure to CeNPs. High-performance liquid chromatography revealed that various organic species released from the yeast were expressed or suppressed after exposure to CeNPs. Although cytotoxicity was not caused by CeNPs, the results of the peptide mass fingerprint analysis of the intracellular protein revealed that Eno2p, a glycolysis enzyme, was expressed after exposure to CeNPs. These results suggest that nanoparticles have the potential to alter microbial metabolism, leading to changes in the compositions of the released substances in the surrounding environment.
AB - To understand the effects of nanoparticles on microorganisms, we experimentally investigated the effects of CeO2 nanoparticles (CeNPs) on yeast (S. cerevisiae) focusing on microbial metabolites and intracellular proteins. The yeast were harvested from a yeast extract peptone dextrose medium containing 0, 10, 100, and 250ppm of CeNPs and incubated for 120h in 1mM NaCl solution at three different pH values: 3, 5, and 7. The yeast released organic matter, P, K, and Mg into the NaCl solution at all pH values, even without CeNPs. Distinct differences were detected in the released organic species and intracellular proteins after exposure to CeNPs. High-performance liquid chromatography revealed that various organic species released from the yeast were expressed or suppressed after exposure to CeNPs. Although cytotoxicity was not caused by CeNPs, the results of the peptide mass fingerprint analysis of the intracellular protein revealed that Eno2p, a glycolysis enzyme, was expressed after exposure to CeNPs. These results suggest that nanoparticles have the potential to alter microbial metabolism, leading to changes in the compositions of the released substances in the surrounding environment.
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U2 - 10.1016/j.chemgeo.2014.10.026
DO - 10.1016/j.chemgeo.2014.10.026
M3 - Article
AN - SCOPUS:84910658419
SN - 0009-2541
VL - 391
SP - 33
EP - 41
JO - Chemical Geology
JF - Chemical Geology
ER -