Effects of cepharanthine alone and in combination with fluoropyrimidine anticancer agent, S-l, on tumor growth of human oral squamous cell carcinoma xenografts in nude mice

Koji Harada, Tarannum Ferdous, Yasutaka Itashiki, Michiyo Takii, Takamichi Mano, Yoshihide Mori, Yoshiya Ueyama

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Background: Chemotherapy has shown little antitumor activity against advanced oral squamous cell carcinoma (OSCC) patients. Therefore, there is an urgent need to develop more effective therapeutic methods for patients with advanced OSCC. Cepharanthine is a biscoclaurine alkaloid extracted from Stephania cepharantha Hayata, which is widely used for the treatment of many acute and chronic diseases, and can exert antitumor effects on several human cancer cells. S-l is a new oral antineoplastic agent that can induce apoptosis in various types of cancer cells, including OSCC. Hence combined treatment of cancer cells with cepharanthine and S-l might exert dramatic antitumor effects on OSCC cells. Materials and Methods: In this study, the response of human OSCC cells to cepharanthine alone and in combination with S-l was examined using nude mouse xenograft models. S-l (10 mg/kg/day, 5 times/week) was administered orally and cepharanthine (20 mg/kg, 5 times/week) was injected into peritumoral tissue for three weeks. Apoptotic cells were detected by a TUNEL method. The protein expression of thymidylate synthase (TS), dihydropyrimidine dehydrogenase (DPD), and orotate phosphoribosyl transferase (OPRT) were assessed using immunohistochemistry; their gene expression was determined using microdissection and RT- PCR, and their protein levels using ELISA. Results: Combined therapy of cepharanthine and S-l exerted.

Original languageEnglish
Pages (from-to)1263-1270
Number of pages8
JournalAnticancer research
Volume29
Issue number4
Publication statusPublished - Apr 1 2009
Externally publishedYes

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Heterografts
Nude Mice
Antineoplastic Agents
Squamous Cell Carcinoma
Growth
Neoplasms
Stephania
Dihydrouracil Dehydrogenase (NADP)
Thymidylate Synthase
Microdissection
In Situ Nick-End Labeling
Acute Disease
Therapeutics
Transferases
Alkaloids
cepharanthine
Proteins
Chronic Disease
Enzyme-Linked Immunosorbent Assay
Immunohistochemistry

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Effects of cepharanthine alone and in combination with fluoropyrimidine anticancer agent, S-l, on tumor growth of human oral squamous cell carcinoma xenografts in nude mice. / Harada, Koji; Ferdous, Tarannum; Itashiki, Yasutaka; Takii, Michiyo; Mano, Takamichi; Mori, Yoshihide; Ueyama, Yoshiya.

In: Anticancer research, Vol. 29, No. 4, 01.04.2009, p. 1263-1270.

Research output: Contribution to journalArticle

Harada, Koji ; Ferdous, Tarannum ; Itashiki, Yasutaka ; Takii, Michiyo ; Mano, Takamichi ; Mori, Yoshihide ; Ueyama, Yoshiya. / Effects of cepharanthine alone and in combination with fluoropyrimidine anticancer agent, S-l, on tumor growth of human oral squamous cell carcinoma xenografts in nude mice. In: Anticancer research. 2009 ; Vol. 29, No. 4. pp. 1263-1270.
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abstract = "Background: Chemotherapy has shown little antitumor activity against advanced oral squamous cell carcinoma (OSCC) patients. Therefore, there is an urgent need to develop more effective therapeutic methods for patients with advanced OSCC. Cepharanthine is a biscoclaurine alkaloid extracted from Stephania cepharantha Hayata, which is widely used for the treatment of many acute and chronic diseases, and can exert antitumor effects on several human cancer cells. S-l is a new oral antineoplastic agent that can induce apoptosis in various types of cancer cells, including OSCC. Hence combined treatment of cancer cells with cepharanthine and S-l might exert dramatic antitumor effects on OSCC cells. Materials and Methods: In this study, the response of human OSCC cells to cepharanthine alone and in combination with S-l was examined using nude mouse xenograft models. S-l (10 mg/kg/day, 5 times/week) was administered orally and cepharanthine (20 mg/kg, 5 times/week) was injected into peritumoral tissue for three weeks. Apoptotic cells were detected by a TUNEL method. The protein expression of thymidylate synthase (TS), dihydropyrimidine dehydrogenase (DPD), and orotate phosphoribosyl transferase (OPRT) were assessed using immunohistochemistry; their gene expression was determined using microdissection and RT- PCR, and their protein levels using ELISA. Results: Combined therapy of cepharanthine and S-l exerted.",
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