Effects of chronic AICAR treatment on fiber composition, enzyme activity, UCP3, and PGC-1 in rat muscles

Masataka Suwa, Hiroshi Nakano, Shuzo Kumagai

    Research output: Contribution to journalArticlepeer-review

    212 Citations (Scopus)

    Abstract

    This study was designed to determine the histological and metabolic effects of the administration of 5′-AMP-activated protein kinase (AMPK) activator 5-aminoimidazole-4-carboxamide-1-β-D-ribofuranoside (AICAR) for 14 successive days. AICAR treatment caused a significant decrease in the percentage of type IIB fibers and the concomitant increase in the percentage of type IIX fibers in extensor digitorum longus (EDL) muscle. The capillary density and the capillary-to-fiber ratio were not altered by AICAR. AICAR treatment increased the glycolytic and oxidative enzyme activities but not the antioxidant enzyme activities. The AICAR treatment increased the uncoupling protein 3 (UCP3) level in EDL and the peroxisome proliferator-activated receptor-γ coactivator-1α protein level in the soleus and EDL muscles, whereas the myogenin level was not altered by AICAR. These results seem to imply that the chronic activation of AMPK alters such muscle histochemical and metabolic characteristics.

    Original languageEnglish
    Pages (from-to)960-968
    Number of pages9
    JournalJournal of Applied Physiology
    Volume95
    Issue number3
    DOIs
    Publication statusPublished - Sep 1 2003

    All Science Journal Classification (ASJC) codes

    • Physiology
    • Physiology (medical)

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