Effects of cold exposure and shear stress on endothelial nitric oxide synthase activation

Kamariah binti Md Isa, Naoto Kawasaki, Keiichi Ueyama, Tateki Sumii, Susumu Kudo

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)


Endothelial nitric oxide synthase (eNOS) is the primary enzyme that produces nitric oxide (NO), which plays an important role in blood vessel relaxation. eNOS activation is stimulated by various mechanical forces, such as shear stress. Several studies have shown that local cooling of the human finger causes strong vasoconstriction, followed after several minutes by cold-induced vasodilation (CIVD). However, the role played by endothelial cells (ECs) in blood vessel regulation in respond to cold temperatures is not fully understood. In this study, we found that low temperature alone does not significantly increase or decrease eNOS activation in ECs. We further found that the combination of shear stress with temperature change leads to a significant increase in eNOS activation at 37 °C and 28 °C, and a decrease at 4 °C. These results show that ECs play an important role in blood vessel regulation under shear stress and low temperature.

Original languageEnglish
Pages (from-to)318-322
Number of pages5
JournalBiochemical and Biophysical Research Communications
Issue number2
Publication statusPublished - Aug 26 2011
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


Dive into the research topics of 'Effects of cold exposure and shear stress on endothelial nitric oxide synthase activation'. Together they form a unique fingerprint.

Cite this