Effects of cold shock treatment on total lipid content and fatty acid composition of Aurantiochytrium limacinum strain mh0186

Yousuke Taoka, Naoki Nagano, Yuji Okita, Hitoshi Izumida, Shinichi Sugimoto, Masahiro Hayashi

Research output: Contribution to journalComment/debate

3 Citations (Scopus)

Abstract

To examine the effect of cold shock treatment on the fatty acid composition of Aurantiochytrium limacinum strain mh0186, a marine thraustochytrid, we cultivated this strain at 28°C for 72 h with shaking and stored the obtained biomass at 10°C for 72 h. A growth experiment was carried out for comparison, wherein strain mh0186 was grown at 10 and 15°C for 72 h with shaking, and it was found that the unsaturation of fatty acids was accelerated relative to that at 28°C. In the cold shock experiment, the total lipid content signifi cantly increased during storage at 10°C for 72 h. Overall, the percentage of unsaturated fatty acids such as docosahexaenoic acid was almost stable while that of n-6 docosapentaenoic acid decreased slightly, but signifi cantly, relative to that in the growth experiment.

Original languageEnglish
Pages (from-to)217-220
Number of pages4
JournalJournal of oleo science
Volume60
Issue number5
DOIs
Publication statusPublished - Jan 1 2011

Fingerprint

Fatty acids
Lipids
Fatty Acids
Chemical analysis
Unsaturated fatty acids
Acids
Docosahexaenoic Acids
Experiments
Unsaturated Fatty Acids
Biomass

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

Effects of cold shock treatment on total lipid content and fatty acid composition of Aurantiochytrium limacinum strain mh0186. / Taoka, Yousuke; Nagano, Naoki; Okita, Yuji; Izumida, Hitoshi; Sugimoto, Shinichi; Hayashi, Masahiro.

In: Journal of oleo science, Vol. 60, No. 5, 01.01.2011, p. 217-220.

Research output: Contribution to journalComment/debate

Taoka, Yousuke ; Nagano, Naoki ; Okita, Yuji ; Izumida, Hitoshi ; Sugimoto, Shinichi ; Hayashi, Masahiro. / Effects of cold shock treatment on total lipid content and fatty acid composition of Aurantiochytrium limacinum strain mh0186. In: Journal of oleo science. 2011 ; Vol. 60, No. 5. pp. 217-220.
@article{feaa0101246d45a5aed251203dcdb86b,
title = "Effects of cold shock treatment on total lipid content and fatty acid composition of Aurantiochytrium limacinum strain mh0186",
abstract = "To examine the effect of cold shock treatment on the fatty acid composition of Aurantiochytrium limacinum strain mh0186, a marine thraustochytrid, we cultivated this strain at 28°C for 72 h with shaking and stored the obtained biomass at 10°C for 72 h. A growth experiment was carried out for comparison, wherein strain mh0186 was grown at 10 and 15°C for 72 h with shaking, and it was found that the unsaturation of fatty acids was accelerated relative to that at 28°C. In the cold shock experiment, the total lipid content signifi cantly increased during storage at 10°C for 72 h. Overall, the percentage of unsaturated fatty acids such as docosahexaenoic acid was almost stable while that of n-6 docosapentaenoic acid decreased slightly, but signifi cantly, relative to that in the growth experiment.",
author = "Yousuke Taoka and Naoki Nagano and Yuji Okita and Hitoshi Izumida and Shinichi Sugimoto and Masahiro Hayashi",
year = "2011",
month = "1",
day = "1",
doi = "10.5650/jos.60.217",
language = "English",
volume = "60",
pages = "217--220",
journal = "Journal of Oleo Science",
issn = "1345-8957",
publisher = "Japan Oil Chemists Society",
number = "5",

}

TY - JOUR

T1 - Effects of cold shock treatment on total lipid content and fatty acid composition of Aurantiochytrium limacinum strain mh0186

AU - Taoka, Yousuke

AU - Nagano, Naoki

AU - Okita, Yuji

AU - Izumida, Hitoshi

AU - Sugimoto, Shinichi

AU - Hayashi, Masahiro

PY - 2011/1/1

Y1 - 2011/1/1

N2 - To examine the effect of cold shock treatment on the fatty acid composition of Aurantiochytrium limacinum strain mh0186, a marine thraustochytrid, we cultivated this strain at 28°C for 72 h with shaking and stored the obtained biomass at 10°C for 72 h. A growth experiment was carried out for comparison, wherein strain mh0186 was grown at 10 and 15°C for 72 h with shaking, and it was found that the unsaturation of fatty acids was accelerated relative to that at 28°C. In the cold shock experiment, the total lipid content signifi cantly increased during storage at 10°C for 72 h. Overall, the percentage of unsaturated fatty acids such as docosahexaenoic acid was almost stable while that of n-6 docosapentaenoic acid decreased slightly, but signifi cantly, relative to that in the growth experiment.

AB - To examine the effect of cold shock treatment on the fatty acid composition of Aurantiochytrium limacinum strain mh0186, a marine thraustochytrid, we cultivated this strain at 28°C for 72 h with shaking and stored the obtained biomass at 10°C for 72 h. A growth experiment was carried out for comparison, wherein strain mh0186 was grown at 10 and 15°C for 72 h with shaking, and it was found that the unsaturation of fatty acids was accelerated relative to that at 28°C. In the cold shock experiment, the total lipid content signifi cantly increased during storage at 10°C for 72 h. Overall, the percentage of unsaturated fatty acids such as docosahexaenoic acid was almost stable while that of n-6 docosapentaenoic acid decreased slightly, but signifi cantly, relative to that in the growth experiment.

UR - http://www.scopus.com/inward/record.url?scp=79955496851&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79955496851&partnerID=8YFLogxK

U2 - 10.5650/jos.60.217

DO - 10.5650/jos.60.217

M3 - Comment/debate

C2 - 21502721

AN - SCOPUS:79955496851

VL - 60

SP - 217

EP - 220

JO - Journal of Oleo Science

JF - Journal of Oleo Science

SN - 1345-8957

IS - 5

ER -