Effects of combinational treatment with ethephon and indole-3-butyric acid on adventitious rooting of Pinus thunbergii cuttings

Yasuhiro Mori, Fumihiko Miyahara, Yuji Tsutsumi, Ryuichiro Kondo

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Adventitious rooting is essential for cutting propagation of pine wilt-resistant Pinus thunbergii. To examine a variety of adventitious rooting potentials among donor plants, cuttings were taken from 31 seedlings within a half-sib family. Rooting abilities of cuttings from each seedling ranged from 0 to 100%. When 11 ortets and 11 ramets (clonally propagated from each ortet) were used as donor plants, there was a positive correlation between rooting abilities of cuttings from ortets and ramets, suggesting that adventitious rooting is dependent on genetic factors in the donor plants. To promote adventitious rooting of cuttings by hormonal treatment, we examined the effect of soaking time in Oxyberon (19.7 mM indole-3-butyric acid (IBA) solution) on rooting. Ten minutes was the best soaking time for rooted cuttings to produce more adventitious roots without impairing normal growth. When cuttings were soaked in Ethrel diluent (69.2 μM ethephon) for 24 h before soaking in Oxyberon for 10 min, a significantly higher rooting ability was observed than those soaked in Oxyberon alone. Ethrel on its own barely affected rooting ability. The positive effect of the combinational treatment was confirmed in a similar experiment using authentic ethephon and IBA instead of Ethrel and Oxyberon. When cuttings were soaked in a mixture of ethephon and silver thiosulfate (STS), an ethylene action inhibitor, before IBA-soaking, the effect was partially diminished compared with combinational treatment without STS. These findings suggest that ethylene action caused by ethephon treatment promotes IBA-mediated adventitious rooting of P. thunbergii cuttings.

Original languageEnglish
Pages (from-to)271-278
Number of pages8
JournalPlant Growth Regulation
Volume63
Issue number3
DOIs
Publication statusPublished - Apr 1 2011

Fingerprint

Pinus thunbergii
Pinus
ethephon
indole butyric acid
rooting
soaking
Seedlings
silver thiosulfate
ethylene
indolebutyric acid
plant cuttings
seedlings
adventitious roots
Growth

All Science Journal Classification (ASJC) codes

  • Physiology
  • Agronomy and Crop Science
  • Plant Science

Cite this

Effects of combinational treatment with ethephon and indole-3-butyric acid on adventitious rooting of Pinus thunbergii cuttings. / Mori, Yasuhiro; Miyahara, Fumihiko; Tsutsumi, Yuji; Kondo, Ryuichiro.

In: Plant Growth Regulation, Vol. 63, No. 3, 01.04.2011, p. 271-278.

Research output: Contribution to journalArticle

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