Effects of copper ion on antioxidant function of Vallisneria asiatica

Aimin Hao, Takahiro Kuba, Yasushi Iseri, Zhenjia Zhang, Yuxian Liu, Tomokazu Haraguchi

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The objective of this research is to demonstrate the antioxidant ecological response of Vallisneria asiatica to the heavy metal Cu2+ pollution which is recently a serious problem in the lakes. Batch experiments with five different concentrations of Cu2+ (0, 5, 10, 20, 40, 80 mg·L-1) were carried out. After the 12th day, the roots and leaves of Vallisneria asiatica dipped in the solution with Cu2+ were removed and the physiological and biochemical indexes, chlorophyll, protein and malondialdehyde (MDA) content, superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) activity were measured. After the experiment; the roots and leaves cultivated in the 0 mg·L-1 of Cu2+ group showed chlorophyll and protein content reduced while the MDA content, POD and SOD activity were increased notoriously. Compared with the 0 mg·L -1 of Cu2+ group, the 5 mg·L-1 of Cu2+ group showed chlorophyll and protein content increase, SOD and POD activity showed also a slight increase, while MDA content decreased. However, for the 10 mg·L-1 of Cu2+ group, the decrease of chlorophyll and protein content and the increase of MDA content were observed. Also a sharp increase of SOD and CAT activity compared with the 5 mg·L-1 group were found; for over 10 mg·L-1 of Cu2+ concentration, chlorophyll content and protein content fell off, whereas increase of MDA became considerable, CAT raised up steadily and POD showed a decrease trend. The results showed that, both insufficiency and overabundance of Cu2+ in water environment can affect the anti-oxidation function of Vallisneria asiatica, which can be observed by the value of SOD, CAT and POD activity. Therefore, the measurement of anti-oxidation activity, protein content and MDA as a forecast and judgment indicator of Cu2+ contaminations in the water environment can be considered in the future.

Original languageEnglish
Pages (from-to)115-122
Number of pages8
JournalEcology and Civil Engineering
Volume14
Issue number2
DOIs
Publication statusPublished - Jan 1 2011

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Antioxidants
antioxidant
Chlorophyll
copper
chlorophyll
Proteins
Copper
protein
ion
Ions
oxidation
Oxidation
Heavy metals
Lakes
effect
Water
Pollution
Contamination
experiment
Experiments

All Science Journal Classification (ASJC) codes

  • Civil and Structural Engineering
  • Ecology

Cite this

Effects of copper ion on antioxidant function of Vallisneria asiatica. / Hao, Aimin; Kuba, Takahiro; Iseri, Yasushi; Zhang, Zhenjia; Liu, Yuxian; Haraguchi, Tomokazu.

In: Ecology and Civil Engineering, Vol. 14, No. 2, 01.01.2011, p. 115-122.

Research output: Contribution to journalArticle

Hao, Aimin ; Kuba, Takahiro ; Iseri, Yasushi ; Zhang, Zhenjia ; Liu, Yuxian ; Haraguchi, Tomokazu. / Effects of copper ion on antioxidant function of Vallisneria asiatica. In: Ecology and Civil Engineering. 2011 ; Vol. 14, No. 2. pp. 115-122.
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abstract = "The objective of this research is to demonstrate the antioxidant ecological response of Vallisneria asiatica to the heavy metal Cu2+ pollution which is recently a serious problem in the lakes. Batch experiments with five different concentrations of Cu2+ (0, 5, 10, 20, 40, 80 mg·L-1) were carried out. After the 12th day, the roots and leaves of Vallisneria asiatica dipped in the solution with Cu2+ were removed and the physiological and biochemical indexes, chlorophyll, protein and malondialdehyde (MDA) content, superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) activity were measured. After the experiment; the roots and leaves cultivated in the 0 mg·L-1 of Cu2+ group showed chlorophyll and protein content reduced while the MDA content, POD and SOD activity were increased notoriously. Compared with the 0 mg·L -1 of Cu2+ group, the 5 mg·L-1 of Cu2+ group showed chlorophyll and protein content increase, SOD and POD activity showed also a slight increase, while MDA content decreased. However, for the 10 mg·L-1 of Cu2+ group, the decrease of chlorophyll and protein content and the increase of MDA content were observed. Also a sharp increase of SOD and CAT activity compared with the 5 mg·L-1 group were found; for over 10 mg·L-1 of Cu2+ concentration, chlorophyll content and protein content fell off, whereas increase of MDA became considerable, CAT raised up steadily and POD showed a decrease trend. The results showed that, both insufficiency and overabundance of Cu2+ in water environment can affect the anti-oxidation function of Vallisneria asiatica, which can be observed by the value of SOD, CAT and POD activity. Therefore, the measurement of anti-oxidation activity, protein content and MDA as a forecast and judgment indicator of Cu2+ contaminations in the water environment can be considered in the future.",
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