セキショウモ (Vallisneria asiatica) の抗酸化機能に及ぼす銅イオンの影響

Translated title of the contribution: Effects of copper ion on antioxidant function of Vallisneria asiatica

? 愛民, 久場 隆広, 井芹 寧, 張 振家, 劉 玉賢, 原口 智和

Research output: Contribution to journalArticle

Abstract

The objective of this research is to demonstrate the antioxdant ecological response of <i>Vallisneria asiatica</i> to the heavy metal Cu<sup>2+</sup> pollution which is recently a serious problem in the lakes. Batch experiments with five different concentrations of Cu<sup>2+</sup> (0, 5, 10, 20, 40, 80 mg· L<sup>-1</sup>) were carried out. After the 12<sup>th</sup> day, the roots and leaves of <i>Vallisneria asiatica</i> dipped in the solution with Cu<sup>2+</sup> were removed and the physiological and biochemical indexes, chlorophyll, protein and malondialdehyde (MDA) content, superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) activity were measured. After the experiment; the roots and leaves cultivated in the 0 mg·L<sup>-1</sup> of Cu<sup>2+</sup> group showed chlorophyll and protein content reduced while the MDA content, POD and SOD activity were increased notoriously. Compared with the 0 mg·L<sup>-1</sup> of Cu<sup>2+</sup> group, the 5 mg·L<sup>-1</sup> of Cu<sup>2+</sup> group showed chlorophyll and protein content increase, SOD and POD activity showed also a slight increase, while MDA content decreased. However, for the 10 mg·L<sup>-1</sup> of Cu<sup>2+</sup> group, the decrease of chlorophyll and protein content and the increase of MDA content were observed. Also a sharp increase of SOD and CAT activity compared with the 5 mg·L<sup>-1</sup> group were found; for over 10 mg·L<sup>-1</sup> of Cu<sup>2+</sup> concentration, chlorophyll content and protein content fell off, whereas increase of MDA became considerable, CAT raised up steadily and POD showed a decrease trend. The results showed that, both insufficiency and overabundance of Cu<sup>2+</sup> in water environment can affect the anti-oxidation function of <i>Vallisneria asiatica</i> which can be observed by the value of SOD, CAT and POD activity. Therefore, the measurement of anti-oxidation activity, protein content and MDA as a forecast and judgment indicator of Cu<sup>2+</sup> contaminations in the water environment can be considered in the future.
Translated title of the contributionEffects of copper ion on antioxidant function of Vallisneria asiatica
Original languageJapanese
Pages (from-to)115-122
Number of pages8
JournalEcology and Civil Engineering
Volume14
Issue number2
DOIs
Publication statusPublished - Dec 31 2011

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