Effects of culture conditions on the mechanical and biological properties of engineered cartilage constructed with collagen hybrid scaffold and human mesenchymal stem cells

Yusuke Nakamuta, Takaaki Arahira, Mitsugu Todo

Research output: Contribution to journalArticle

Abstract

Abstract: Mesenchymal stem cells (MSCs) has been used as one of the new cell sources in osteochondral tissue engineering. It has been well known that control of their differentiation into chondrocytes plays a key role in developing engineered cartilages. Therefore, this study aims to develop a fundamental protocol to control the differentiation and proliferation of MSCs to construct an engineered cartilage. We compared the effects of three different culture conditions on cell proliferation, extracellular matrix formation and the mechanical response of engineered cartilage constructed using a collagen-based hybrid scaffold and human MSCs. The experimental results clearly showed that the combined culture condition of the chondrogenic differentiation culture and the chondrocyte growth culture exhibited statistically significant cell proliferation, ECM formation and stiffness responses as compared to the other two combinations. It is thus concluded that the combination of the differentiation culture with the subsequent growth culture is recommended as the culture condition for chondrogenic tissue engineering using hMSCs. [Figure not available: see fulltext.]

Original languageEnglish
Article number119
JournalJournal of Materials Science: Materials in Medicine
Volume30
Issue number10
DOIs
Publication statusPublished - Oct 1 2019

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Cartilage
Scaffolds (biology)
Stem cells
Mesenchymal Stromal Cells
Collagen
Scaffolds
Cell proliferation
Tissue Engineering
Chondrocytes
Tissue engineering
Cell culture
Cell Proliferation
Military electronic countermeasures
Growth
Extracellular Matrix
Stiffness

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering

Cite this

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