Effects of Derinat on ischemia-reperfusion-induced pressure ulcer mouse model

Jiadai Liu, Elena G. Rybakina, Eelena A. Korneva, Mami Noda

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Sodium salt of deoxyribonucleic acid (DNA), Derinat, isolated from the soft roes of Russian sturgeon, has been utilized as an immunomodulator for the treatment of reactive oxygen species (ROS)-associated diseases in clinics. Here we show that treatment with Derinat has an anti-inflammatory and anti-oxidative effects on cutaneous ischemia-reperfusion (IR) injury in pressure ulcer (PU) model mice. Dorsal skin damage and dermal edema in mild PU model mice were attenuated by treatment with Derinat. Immunohistochemical and biochemical analyses showed that Derinat suppressed IR-induced oxidative damage, i.e. accumulation of 8-hydroxy-2′-deoxyguanosine (8-OHdG), and related inflammatory factors such as cyclooxygenase 2 (COX-2) and IL-6 receptor (IL-6R) in dorsal skin from PU model mice. We also verified that phospholyated/non-phosphorylated ratio of extracellular signal-regulated kinase (Erk) and p38 mitogen-activated protein kinase (MAPK) increased after IR, which were attenuated by Derinat. We then compared the effect of Derinat with that of salmon DNA and other PU therapeutic agents, prostaglandin E1 (PGE1) and basic fibroblast growth factor (bFGF), by using severe PU model mice. The effects of Derinat and salmon-DNA were compatible with those of PGE1 and bFGF. These results suggest that Derinat other fish-derived DNA formulation could be effective enough and become intriguing new therapeutic options.

Original languageEnglish
Pages (from-to)123-130
Number of pages8
JournalJournal of Pharmacological Sciences
Volume138
Issue number2
DOIs
Publication statusPublished - Oct 2018

Fingerprint

Pressure Ulcer
Reperfusion
Ischemia
Alprostadil
Salmon
DNA
Fibroblast Growth Factor 2
Skin
Skin Ulcer
Interleukin-6 Receptors
Derinat
Extracellular Signal-Regulated MAP Kinases
Immunologic Factors
p38 Mitogen-Activated Protein Kinases
Cyclooxygenase 2
Reperfusion Injury
Reactive Oxygen Species
Edema
Fishes
Anti-Inflammatory Agents

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Pharmacology

Cite this

Effects of Derinat on ischemia-reperfusion-induced pressure ulcer mouse model. / Liu, Jiadai; Rybakina, Elena G.; Korneva, Eelena A.; Noda, Mami.

In: Journal of Pharmacological Sciences, Vol. 138, No. 2, 10.2018, p. 123-130.

Research output: Contribution to journalArticle

Liu, Jiadai ; Rybakina, Elena G. ; Korneva, Eelena A. ; Noda, Mami. / Effects of Derinat on ischemia-reperfusion-induced pressure ulcer mouse model. In: Journal of Pharmacological Sciences. 2018 ; Vol. 138, No. 2. pp. 123-130.
@article{7954c2e608f24e0bbcd017562e9789aa,
title = "Effects of Derinat on ischemia-reperfusion-induced pressure ulcer mouse model",
abstract = "Sodium salt of deoxyribonucleic acid (DNA), Derinat, isolated from the soft roes of Russian sturgeon, has been utilized as an immunomodulator for the treatment of reactive oxygen species (ROS)-associated diseases in clinics. Here we show that treatment with Derinat has an anti-inflammatory and anti-oxidative effects on cutaneous ischemia-reperfusion (IR) injury in pressure ulcer (PU) model mice. Dorsal skin damage and dermal edema in mild PU model mice were attenuated by treatment with Derinat. Immunohistochemical and biochemical analyses showed that Derinat suppressed IR-induced oxidative damage, i.e. accumulation of 8-hydroxy-2′-deoxyguanosine (8-OHdG), and related inflammatory factors such as cyclooxygenase 2 (COX-2) and IL-6 receptor (IL-6R) in dorsal skin from PU model mice. We also verified that phospholyated/non-phosphorylated ratio of extracellular signal-regulated kinase (Erk) and p38 mitogen-activated protein kinase (MAPK) increased after IR, which were attenuated by Derinat. We then compared the effect of Derinat with that of salmon DNA and other PU therapeutic agents, prostaglandin E1 (PGE1) and basic fibroblast growth factor (bFGF), by using severe PU model mice. The effects of Derinat and salmon-DNA were compatible with those of PGE1 and bFGF. These results suggest that Derinat other fish-derived DNA formulation could be effective enough and become intriguing new therapeutic options.",
author = "Jiadai Liu and Rybakina, {Elena G.} and Korneva, {Eelena A.} and Mami Noda",
year = "2018",
month = "10",
doi = "10.1016/j.jphs.2018.08.013",
language = "English",
volume = "138",
pages = "123--130",
journal = "Journal of Pharmacological Sciences",
issn = "1347-8613",
publisher = "Japanese Pharmacological Society",
number = "2",

}

TY - JOUR

T1 - Effects of Derinat on ischemia-reperfusion-induced pressure ulcer mouse model

AU - Liu, Jiadai

AU - Rybakina, Elena G.

AU - Korneva, Eelena A.

AU - Noda, Mami

PY - 2018/10

Y1 - 2018/10

N2 - Sodium salt of deoxyribonucleic acid (DNA), Derinat, isolated from the soft roes of Russian sturgeon, has been utilized as an immunomodulator for the treatment of reactive oxygen species (ROS)-associated diseases in clinics. Here we show that treatment with Derinat has an anti-inflammatory and anti-oxidative effects on cutaneous ischemia-reperfusion (IR) injury in pressure ulcer (PU) model mice. Dorsal skin damage and dermal edema in mild PU model mice were attenuated by treatment with Derinat. Immunohistochemical and biochemical analyses showed that Derinat suppressed IR-induced oxidative damage, i.e. accumulation of 8-hydroxy-2′-deoxyguanosine (8-OHdG), and related inflammatory factors such as cyclooxygenase 2 (COX-2) and IL-6 receptor (IL-6R) in dorsal skin from PU model mice. We also verified that phospholyated/non-phosphorylated ratio of extracellular signal-regulated kinase (Erk) and p38 mitogen-activated protein kinase (MAPK) increased after IR, which were attenuated by Derinat. We then compared the effect of Derinat with that of salmon DNA and other PU therapeutic agents, prostaglandin E1 (PGE1) and basic fibroblast growth factor (bFGF), by using severe PU model mice. The effects of Derinat and salmon-DNA were compatible with those of PGE1 and bFGF. These results suggest that Derinat other fish-derived DNA formulation could be effective enough and become intriguing new therapeutic options.

AB - Sodium salt of deoxyribonucleic acid (DNA), Derinat, isolated from the soft roes of Russian sturgeon, has been utilized as an immunomodulator for the treatment of reactive oxygen species (ROS)-associated diseases in clinics. Here we show that treatment with Derinat has an anti-inflammatory and anti-oxidative effects on cutaneous ischemia-reperfusion (IR) injury in pressure ulcer (PU) model mice. Dorsal skin damage and dermal edema in mild PU model mice were attenuated by treatment with Derinat. Immunohistochemical and biochemical analyses showed that Derinat suppressed IR-induced oxidative damage, i.e. accumulation of 8-hydroxy-2′-deoxyguanosine (8-OHdG), and related inflammatory factors such as cyclooxygenase 2 (COX-2) and IL-6 receptor (IL-6R) in dorsal skin from PU model mice. We also verified that phospholyated/non-phosphorylated ratio of extracellular signal-regulated kinase (Erk) and p38 mitogen-activated protein kinase (MAPK) increased after IR, which were attenuated by Derinat. We then compared the effect of Derinat with that of salmon DNA and other PU therapeutic agents, prostaglandin E1 (PGE1) and basic fibroblast growth factor (bFGF), by using severe PU model mice. The effects of Derinat and salmon-DNA were compatible with those of PGE1 and bFGF. These results suggest that Derinat other fish-derived DNA formulation could be effective enough and become intriguing new therapeutic options.

UR - http://www.scopus.com/inward/record.url?scp=85055110277&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85055110277&partnerID=8YFLogxK

U2 - 10.1016/j.jphs.2018.08.013

DO - 10.1016/j.jphs.2018.08.013

M3 - Article

C2 - 30360946

AN - SCOPUS:85055110277

VL - 138

SP - 123

EP - 130

JO - Journal of Pharmacological Sciences

JF - Journal of Pharmacological Sciences

SN - 1347-8613

IS - 2

ER -