Effects of donor age and contamination level of dioxins and related chemicals on frequency of sister chromatid exchanges in human lymphocytes cultured in vitro

Junya Nagayama, Mayumi Nagayama, Takao Iida, Hironori Hirakawa, Takahiko Matsueda, Masafumi Ohki, Hiroshi Tsuji

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Frequencies of sister chromatid exchanges (SCEs) of human lymphocytes in control (DMSO treated) and 7,8-benzoflavone (ANF) treated cultures were measured in 39 healthy Japanese people and examined in connection with donor age. Both the control (baseline) and ANF induced SCE rates were significantly enhanced with age and highly positive correlation was observed between them. Therefore, in vivo aging seemed to have some effects on the frequencies of SCEs in human lymphocytes cultured in vitro. Concentrations of PCDDs, PCDFs and Co-PCBs in the blood and sebum of face were determined in the same Japanese subjects. Significantly positive correlation was observed between the blood and sebum in their total TEQ levels. Hence, PCDDs, PCDFs and Co-PCBs, which have been contaminating human bodies, are considered proportionally excreted from the sebum of face or body. Their total TEQ concentrations were also meaningfully increased with donor age in the sebum of face as well as in the blood. Either the baseline or ANF induced SCE frequencies was not enhanced with the total TEQ levels in the blood. Therefore, background levels of their contamination seem not to affect the SCE rates of the lymphocytes in the control and ANF treated cultures.

Original languageEnglish
Pages (from-to)845-849
Number of pages5
JournalChemosphere
Volume43
Issue number4-7
DOIs
Publication statusPublished - May 16 2001

Fingerprint

Sister Chromatid Exchange
Dioxins
Lymphocytes
Sebum
Atrial Natriuretic Factor
dioxin
Contamination
Blood
blood
Tissue Donors
Polychlorinated Biphenyls
PCDF
PCDD
Polychlorinated biphenyls
exchange rate
PCB
background level
Dimethyl Sulfoxide
Cell culture
Human Body

All Science Journal Classification (ASJC) codes

  • Environmental Engineering
  • Environmental Chemistry
  • Chemistry(all)
  • Pollution
  • Health, Toxicology and Mutagenesis

Cite this

Effects of donor age and contamination level of dioxins and related chemicals on frequency of sister chromatid exchanges in human lymphocytes cultured in vitro. / Nagayama, Junya; Nagayama, Mayumi; Iida, Takao; Hirakawa, Hironori; Matsueda, Takahiko; Ohki, Masafumi; Tsuji, Hiroshi.

In: Chemosphere, Vol. 43, No. 4-7, 16.05.2001, p. 845-849.

Research output: Contribution to journalArticle

Nagayama, Junya ; Nagayama, Mayumi ; Iida, Takao ; Hirakawa, Hironori ; Matsueda, Takahiko ; Ohki, Masafumi ; Tsuji, Hiroshi. / Effects of donor age and contamination level of dioxins and related chemicals on frequency of sister chromatid exchanges in human lymphocytes cultured in vitro. In: Chemosphere. 2001 ; Vol. 43, No. 4-7. pp. 845-849.
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