TY - JOUR
T1 - Effects of Epigallocatechin Gallate on Viability and Cellular Proteins of Staphylococcus aureus
AU - Kitichalermkiat, Apisada
AU - Kurahachi, Masahiro
AU - Nonaka, Ai
AU - Nakayama, Motokazu
AU - Shimatani, Kanami
AU - Shigemune, Naofumi
AU - Tsugukuni, Takashi
AU - Hitomi, Jun
AU - Sato, Jun
AU - Sonoda, Takumi
AU - Masuda, Yoshimitsu
AU - Honjoh, Ken ichi
AU - Miyamoto, Takahisa
N1 - Publisher Copyright:
Copyright © 2019, Japanese Society for Food Science and Technology
PY - 2019
Y1 - 2019
N2 - This study investigated the effect of epigallocatechin gallate (EGCg) on Staphylococcus aureus to determine its mechanism of antibacterial action. Adsorption of EGCg on the cell envelope of S. aureus after EGCg treatment was demonstrated using a FITC-labeled antibody specific to EGCg. After EGCg treatment of S. aureus for 4 h, abnormalities in septum formation and cell segregation were observed at concentrations greater than 250 mg/L, and debris presumed to arise from cell destruction or leakage of cytoplasmic materials was observed around the cells at 500 mg/L. Two-dimensional electrophoresis of proteins prepared from EGCg-treated S. aureus cells revealed the presence of 18 protein spots that disappeared or showed markedly decreased intensity compared to those from control cells. These proteins included DnaK, elongation factor G, DNA-directed RNA polymerase, l-lactate dehydrogenase, pyruvate dehydrogenase, and acetate kinase. Furthermore, S. aureus showed decreased glucose uptake after EGCg treatment. These results suggest that EGCg inhibits the functions of cell-envelope proteins, and it causes cellular damage and disruption of the cells in S. aureus.
AB - This study investigated the effect of epigallocatechin gallate (EGCg) on Staphylococcus aureus to determine its mechanism of antibacterial action. Adsorption of EGCg on the cell envelope of S. aureus after EGCg treatment was demonstrated using a FITC-labeled antibody specific to EGCg. After EGCg treatment of S. aureus for 4 h, abnormalities in septum formation and cell segregation were observed at concentrations greater than 250 mg/L, and debris presumed to arise from cell destruction or leakage of cytoplasmic materials was observed around the cells at 500 mg/L. Two-dimensional electrophoresis of proteins prepared from EGCg-treated S. aureus cells revealed the presence of 18 protein spots that disappeared or showed markedly decreased intensity compared to those from control cells. These proteins included DnaK, elongation factor G, DNA-directed RNA polymerase, l-lactate dehydrogenase, pyruvate dehydrogenase, and acetate kinase. Furthermore, S. aureus showed decreased glucose uptake after EGCg treatment. These results suggest that EGCg inhibits the functions of cell-envelope proteins, and it causes cellular damage and disruption of the cells in S. aureus.
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U2 - 10.3136/fstr.25.277
DO - 10.3136/fstr.25.277
M3 - Article
AN - SCOPUS:85065541479
SN - 1344-6606
VL - 25
SP - 277
EP - 285
JO - Food Science and Technology Research
JF - Food Science and Technology Research
IS - 2
ER -