Effects of Epigallocatechin Gallate on Viability and Cellular Proteins of Staphylococcus aureus

Apisada Kitichalermkiat, Masahiro Kurahachi, Ai Nonaka, Motokazu Nakayama, Kanami Shimatani, Naofumi Shigemune, Takashi Tsugukuni, Jun Hitomi, Jun Sato, Takumi Sonoda, Yoshimitsu Masuda, Ken-Ichi Honjoh, Takahisa Miyamoto

Research output: Contribution to journalArticle

Abstract

This study investigated the effect of epigallocatechin gallate (EGCg) on Staphylococcus aureus to determine its mechanism of antibacterial action. Adsorption of EGCg on the cell envelope of S. aureus after EGCg treatment was demonstrated using a FITC-labeled antibody specific to EGCg. After EGCg treatment of S. aureus for 4 h, abnormalities in septum formation and cell segregation were observed at concentrations greater than 250 mg/L, and debris presumed to arise from cell destruction or leakage of cytoplasmic materials was observed around the cells at 500 mg/L. Two-dimensional electrophoresis of proteins prepared from EGCg-treated S. aureus cells revealed the presence of 18 protein spots that disappeared or showed markedly decreased intensity compared to those from control cells. These proteins included DnaK, elongation factor G, DNA-directed RNA polymerase, l-lactate dehydrogenase, pyruvate dehydrogenase, and acetate kinase. Furthermore, S. aureus showed decreased glucose uptake after EGCg treatment. These results suggest that EGCg inhibits the functions of cell-envelope proteins, and it causes cellular damage and disruption of the cells in S. aureus.

Original languageEnglish
Pages (from-to)277-285
Number of pages9
JournalFood Science and Technology Research
Volume25
Issue number2
DOIs
Publication statusPublished - Jan 1 2019

Fingerprint

epigallocatechin
Staphylococcus aureus
viability
Proteins
proteins
cells
Electrophoresis
RNA
Debris
Antibodies
Glucose
Elongation
acetate kinase
DNA
pyruvate dehydrogenase (acetyl-transferring) kinase
Acetate Kinase
Peptide Elongation Factor G
Adsorption
epigallocatechin gallate
Viability

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Chemical Engineering(all)
  • Industrial and Manufacturing Engineering
  • Marketing

Cite this

Effects of Epigallocatechin Gallate on Viability and Cellular Proteins of Staphylococcus aureus. / Kitichalermkiat, Apisada; Kurahachi, Masahiro; Nonaka, Ai; Nakayama, Motokazu; Shimatani, Kanami; Shigemune, Naofumi; Tsugukuni, Takashi; Hitomi, Jun; Sato, Jun; Sonoda, Takumi; Masuda, Yoshimitsu; Honjoh, Ken-Ichi; Miyamoto, Takahisa.

In: Food Science and Technology Research, Vol. 25, No. 2, 01.01.2019, p. 277-285.

Research output: Contribution to journalArticle

Kitichalermkiat, A, Kurahachi, M, Nonaka, A, Nakayama, M, Shimatani, K, Shigemune, N, Tsugukuni, T, Hitomi, J, Sato, J, Sonoda, T, Masuda, Y, Honjoh, K-I & Miyamoto, T 2019, 'Effects of Epigallocatechin Gallate on Viability and Cellular Proteins of Staphylococcus aureus', Food Science and Technology Research, vol. 25, no. 2, pp. 277-285. https://doi.org/10.3136/fstr.25.277
Kitichalermkiat A, Kurahachi M, Nonaka A, Nakayama M, Shimatani K, Shigemune N et al. Effects of Epigallocatechin Gallate on Viability and Cellular Proteins of Staphylococcus aureus. Food Science and Technology Research. 2019 Jan 1;25(2):277-285. https://doi.org/10.3136/fstr.25.277
Kitichalermkiat, Apisada ; Kurahachi, Masahiro ; Nonaka, Ai ; Nakayama, Motokazu ; Shimatani, Kanami ; Shigemune, Naofumi ; Tsugukuni, Takashi ; Hitomi, Jun ; Sato, Jun ; Sonoda, Takumi ; Masuda, Yoshimitsu ; Honjoh, Ken-Ichi ; Miyamoto, Takahisa. / Effects of Epigallocatechin Gallate on Viability and Cellular Proteins of Staphylococcus aureus. In: Food Science and Technology Research. 2019 ; Vol. 25, No. 2. pp. 277-285.
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