Effects of epithelial cell supernatant on membrane potential and contraction of dog airway smooth muscles.

Koichiro Matsumoto, H. Aizawa, R. Inoue, S. Hamano, S. Ikeda, Z. Xie, Masato Hirata, N. Hara, Y. Ito

Research output: Contribution to journalArticle

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Abstract

We investigated the effects of cultured epithelial cells and supernatants on resting membrane potential and excitatory neuroeffector transmission in smooth muscle cells of dog trachea and bronchioles. The mean resting membrane potential of the mucosa-free tracheal smooth muscle cells was -59.5 +/- 1.5 mV (+/- SD). Application of cultured epithelial cells (> 2.5 x 10(5) cells/ml) hyperpolarized the membrane, resulting in a potential of -64.5 +/- 1.7 mV. The supernatant of the cultured epithelial cells also increased the resting membrane potential of the mucosa-free tracheal smooth muscle cells by 4 to 9 mV. These hyperpolarizing actions were not modified by indomethacin (10(-5) M), l-NG-nitroarginine (10(-5) M), or oxyhemoglobin (10(-5) M), but were inhibited by glibenclamide (10(-6) M). The supernatants of the cultured epithelial cells completely or partially suppressed the contractile response of epithelium-denuded bronchioles to electrical field stimulations and suppressed the amplitude of excitatory junction potentials of the trachealis evoked by electrical field stimulations. Indomethacin prevented the inhibitory effect of supernatants on the amplitude of twitch contractions and excitatory junction potentials and markedly suppressed supernatant-associated inhibition of the excitatory neuroeffector transmission. Furthermore, indomethacin with AA861, a lipoxygenase inhibitor, completely suppressed this effect. Our findings suggest that cultured airway epithelial cells spontaneously release at least two factors. One factor selectively modulates the resting membrane potential, and the other inhibits the excitatory neuroeffector transmission.

Original languageEnglish
Pages (from-to)322-330
Number of pages9
JournalAmerican journal of respiratory cell and molecular biology
Volume10
Issue number3
DOIs
Publication statusPublished - Jan 1 1994

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Membrane Potentials
Smooth Muscle
Muscle
Epithelial Cells
Dogs
Cultured Cells
Membranes
Indomethacin
Smooth Muscle Myocytes
Bronchioles
Cells
Electric Stimulation
Mucous Membrane
Lipoxygenase Inhibitors
Oxyhemoglobins
Glyburide
Nitroarginine
Trachea
Evoked Potentials
Epithelium

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Pulmonary and Respiratory Medicine
  • Clinical Biochemistry
  • Cell Biology

Cite this

Effects of epithelial cell supernatant on membrane potential and contraction of dog airway smooth muscles. / Matsumoto, Koichiro; Aizawa, H.; Inoue, R.; Hamano, S.; Ikeda, S.; Xie, Z.; Hirata, Masato; Hara, N.; Ito, Y.

In: American journal of respiratory cell and molecular biology, Vol. 10, No. 3, 01.01.1994, p. 322-330.

Research output: Contribution to journalArticle

Matsumoto, Koichiro ; Aizawa, H. ; Inoue, R. ; Hamano, S. ; Ikeda, S. ; Xie, Z. ; Hirata, Masato ; Hara, N. ; Ito, Y. / Effects of epithelial cell supernatant on membrane potential and contraction of dog airway smooth muscles. In: American journal of respiratory cell and molecular biology. 1994 ; Vol. 10, No. 3. pp. 322-330.
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