Effects of halothane on the intracellular Ca2+ concentration of isolated guinea pig hepatocytes

H. Kinugawa, M. Kawahara, M. Sugimura, Tohru Takarada, K. Fujii, O. Yuge

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Abstract

The effects of halothane on the intracellular Ca2+ concentration ([Ca2+](i)) of isolated guinea pig hepatocytes were investigated. When hepatocytes were incubated in Hank's solution containing Ca2+, trypan blue uptake which indicates death of cell was 13.5 ± 3.41%, and [Ca2+](i) was 158 ± 73.5 nM in control. By halothane exposure, both trypan blue uptake and [Ca2+](i) were increased in concentration dependent fashion (Fig. 2, 3). When hepatocytes were incubated in Hank's solution excluded Ca2+, trypan blue uptake was 31.2 ± 6.40%, and [Ca2+](i) was 249 ± 27.5 nM in control. By halothane exposure, trypan blue uptake was increased in concentration dependent fashion, while [Ca2+](i) was decreased (Fig. 4, 5). From these results, the increase of trypan blue uptake in experiment 1 and 2 indicate the hepatotoxicity of halothane with or without Ca2+ in medium. The increase of [Ca2+](i) of hepatocytes in physiological condition indicate the direct injury of halothane to hepatocyte cell membrane.

Original languageEnglish
Pages (from-to)490-494
Number of pages5
JournalJournal of Japanese Dental Society of Anesthesiology
Volume23
Issue number3
Publication statusPublished - Jan 1 1995
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Dentistry(all)
  • Anesthesiology and Pain Medicine

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