Effects of high-pressure carbonation on intracellular ATP and NADH levels and DNA damage in Escherichia coli cells

Wannaporn Klangpetch, Seiji Noma, Noriyuki Igura, Mitsuya Shimoda

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In order to understand the microbial inactivation mechanism of high-pressure carbonation (HPC), we examined the changes in the activity of the respiratory chain and DNA damage in Escherichia coli cells. HPC was performed under 1-6 MPa at 30°C for 1 min. The increase in CO2 pressure decreased the number of viable cells of E. coli, intracellular ATP, and intracellular NADH, and increased the number of apurinic/apyrimidinic sites. These results indicate that HPC has a detrimental effect on the functioning of the respiratory chain in E. coli and induces DNA damage, which could result in the death of the bacterial cells.

Original languageEnglish
Pages (from-to)199-203
Number of pages5
JournalBiocontrol Science
Volume18
Issue number4
DOIs
Publication statusPublished - 2013

All Science Journal Classification (ASJC) codes

  • Applied Microbiology and Biotechnology
  • Public Health, Environmental and Occupational Health

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