Effects of hormones and osmolarity in the culture medium on germinal vesicle breakdown of porcine oocytes

Nobuhiko Yamauchi, H. Sasada, E. Soloy, T. Dominko, K. Kikuchi, T. Nagai

Research output: Contribution to journalArticle

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Abstract

The present study was conducted to examine effects of hormones and osmolarity on germinal vesicle breakdown (GVBD) and histone H1 kinase (H1K) activity in porcine oocytes cultured in vitro. The basic medium used for culture of oocytes was modified Tyrode's solution in which the osmolarity was adjusted to 134 to 495 mOsm by changing the concentration of sodium chloride (NaCl). When the hormones were present, osmolarity of medium that allows GVBD of oocytes was less than 400 mOsm. However, the range of osmolarity of medium that allows meiotic maturation of oocytes was 210 to 362 mOsm. On the other hand, without hormonal supplement, the incidence of GVBD in oocytes decreased as the osmolarity of the medium increased in the rage of 210 to 362 mOsm. By increasing the osmolarity of the medium from 210 to 362 mOsm by addition with sorbitol instead of NaCl, the incidence decreased from 89.1% to 13.3%. In oocytes cultured in medium of 210 mOsm without hormones, the percentage of oocytes that underwent GVBD and had increased H1K activity 20 h after culture was significantly higher (P<0.05) than those of oocytes cultured in the same medium supplemented with hormones or medium of 362 mOsm. These results indicate that in vitro induction of GVBD in porcine oocytes is strongly affected by osmolarity of the medium in the absence of hormones. The results also suggest that under low osmolarity (210 mOsm), GVBD is accelerated with rapid increase of H1K activity.

Original languageEnglish
Pages (from-to)153-162
Number of pages10
JournalTheriogenology
Volume52
Issue number1
DOIs
Publication statusPublished - Jul 1 1999

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germinal vesicle
osmolarity
Osmolar Concentration
Oocytes
Culture Media
oocytes
Swine
culture media
hormones
Hormones
swine
sodium chloride
phosphotransferases (kinases)
Phosphotransferases
Rage
incidence
Sorbitol
Incidence
sorbitol
histones

All Science Journal Classification (ASJC) codes

  • Small Animals
  • Food Animals
  • Animal Science and Zoology
  • Equine

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Effects of hormones and osmolarity in the culture medium on germinal vesicle breakdown of porcine oocytes. / Yamauchi, Nobuhiko; Sasada, H.; Soloy, E.; Dominko, T.; Kikuchi, K.; Nagai, T.

In: Theriogenology, Vol. 52, No. 1, 01.07.1999, p. 153-162.

Research output: Contribution to journalArticle

Yamauchi, Nobuhiko ; Sasada, H. ; Soloy, E. ; Dominko, T. ; Kikuchi, K. ; Nagai, T. / Effects of hormones and osmolarity in the culture medium on germinal vesicle breakdown of porcine oocytes. In: Theriogenology. 1999 ; Vol. 52, No. 1. pp. 153-162.
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