Effects of Host Cell Density on Cell Infection Level in Antheraea eucalypti (Lepidoptera: Saturniidae) Cell Cultures Persistently Infected with Nosema bombycis (Microsporida: Nosematidae)

Chisa Yasunaga-Aoki, MASAKO FUNAKOSHI, TAKESHI KAWARABATA

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

ABSTRACT. Spores of Nosema bombycis Y9101, isolated from the beet armyworm, Spodoptera exigua, were primed with an alkaline solution and inoculated into Antheraea eucalypti cell cultures. Infected cells were subcultured every five days at three cell densities (2.5 × 103, 5.0 × 103, and 1.0 × 104 cells/cm2). A difference was observed in the spread of N. bombycis Y9101 infection between low‐density and higher‐density cultures of host cells. The host cell density did not affect the productivity of secondary infective forms of the parasite. The principal factor determining the rate of microsporidian infection in vitro was the number of host cells existing within the reach of extruded short‐coiled polar tubes from spores germinated intracellularly.

Original languageEnglish
Pages (from-to)133-137
Number of pages5
JournalJournal of Eukaryotic Microbiology
Volume41
Issue number2
DOIs
Publication statusPublished - Jan 1 1994

All Science Journal Classification (ASJC) codes

  • Microbiology

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