Effects of hydrothermal treatment with CaCl2 solution on surface property and cell response of titanium implants

M. Nakagawa, L. Zhang, K. Udoh, S. Matsuya, K. Ishikawa

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55 Citations (Scopus)


In order to obtain early and good osteointegration after implantation of a titanium implant in the human body, the surface modified treatments using NaOH or H2O2 etc. were reported. In this study, titanium was hydrothermally treated with CaCl2 solutions at 200°C for 24hr (CaCl2-HT). Scanning electron microscope (SEM) observation clearly showed apatite deposition on the surface of CaCl2 HT treated titanium faster than other chemical treated titanium immersion in simulated body fluid. X-ray photoelectron spectroscopy (XPS) analysis demonstrated that Ti-O-Ca bonding was formed on titanium surface by hydrothermal treatment with CaCl 2 solution. And it was revealed that thickness of TiO2, which was known to play important roles for the formation of bone-like apatite, became approximately three times thicker than as-polished titanium. The amount of initial attached MC3T3-E1 cells on as-polished and NaOH, H2O 2 and this CaCl2 HT treated titanium were almost the same values. After 5 days incubation, the growth rate of MC3T3-E1 cells on CaCl 2-HT treated titanium was significantly higher than that on other chemical treated titanium. The hydrothermal treatment with 10-20 mmol/L CaCl2 solution at 200°C was an effective method for the fabrication of titanium implant with good bioactivity and osteoconductivity.

Original languageEnglish
Pages (from-to)985-991
Number of pages7
JournalJournal of Materials Science: Materials in Medicine
Issue number11
Publication statusPublished - Nov 2005

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering


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