Effects of immunization against VIP on neurotransmission in cat trachea

H. Hakoda, X. Zhouqiu, H. Aizawa, H. Inoue, M. Hirata, Y. Ito

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

To examine the possible role of vasoactive intestinal polypeptide (VIP) in excitatory and inhibitory neurotransmission and physiological function of coexistence VIP and acetylcholine at vagus nerve terminals in the cat trachea, we immunized five cats each against conjugate of VIP-bovine serum albumin (BSA) and BSA, ely. A booster injection of VIP-BSA given 3 wk after the primary one elevated antibodies against VIP, as detected by enzyme-linked immunosorbent assay, but not in the control cats identically injected with BSA. After immunization, in vitro, summation phenomena observed in the amplitude of contractions and excitatory junction potentials of the trachea evoked by repetitive field stimulation were markedly enhanced, whereas the amplitude of phasic relaxation evoked by repetitive field stimulation in the presence of serotonin, atropine, and guanethidine was markedly reduced. In contrast, in vivo, the change in pulmonary resistance evoked by vagal stimulation was not affected in the immunized cats. We conclude that antibodies against neurotransmitter VIP are a potential modulatory factor in excitatory and inhibitory neurotransmission in airway smooth muscle at tissue or cellular levels. The lack of correlation between in vivo and in vitro conditions was discussed in relation to VIP autoantibodies in normal and asthmatic humans.

Original languageEnglish
Pages (from-to)L341-L348
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume261
Issue number4 5-2
Publication statusPublished - Jan 1 1991

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Vasoactive Intestinal Peptide
Trachea
Synaptic Transmission
Immunization
Cats
Bovine Serum Albumin
Guanethidine
Vagus Nerve
Antibodies
Atropine
Evoked Potentials
Autoantibodies
Acetylcholine
Smooth Muscle
Neurotransmitter Agents
Serotonin
Enzyme-Linked Immunosorbent Assay
Muscles
Lung
Injections

All Science Journal Classification (ASJC) codes

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology

Cite this

Hakoda, H., Zhouqiu, X., Aizawa, H., Inoue, H., Hirata, M., & Ito, Y. (1991). Effects of immunization against VIP on neurotransmission in cat trachea. American Journal of Physiology - Lung Cellular and Molecular Physiology, 261(4 5-2), L341-L348.

Effects of immunization against VIP on neurotransmission in cat trachea. / Hakoda, H.; Zhouqiu, X.; Aizawa, H.; Inoue, H.; Hirata, M.; Ito, Y.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 261, No. 4 5-2, 01.01.1991, p. L341-L348.

Research output: Contribution to journalArticle

Hakoda, H, Zhouqiu, X, Aizawa, H, Inoue, H, Hirata, M & Ito, Y 1991, 'Effects of immunization against VIP on neurotransmission in cat trachea', American Journal of Physiology - Lung Cellular and Molecular Physiology, vol. 261, no. 4 5-2, pp. L341-L348.
Hakoda, H. ; Zhouqiu, X. ; Aizawa, H. ; Inoue, H. ; Hirata, M. ; Ito, Y. / Effects of immunization against VIP on neurotransmission in cat trachea. In: American Journal of Physiology - Lung Cellular and Molecular Physiology. 1991 ; Vol. 261, No. 4 5-2. pp. L341-L348.
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