Effects of Kurozu concentrated liquid on adipocyte size in rats

Li Tao Tong, Yoshinori Katakura, Sayaka Kawamura, Sanae Baba, Yasutake Tanaka, Miyako Udono, Yoshie Kondo, Kumi Nakamura, Katsumi Imaizumi, Masao Sato

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Background. Kurozu concentrated liquid (KCL) is used as a health-promoting supplement for the treatment of disorders such as cancer, hyperlipidemia, and hypertension in Japan. We investigated the possible anti-obesity effects of KCL in rats. Methods. Male Sprague Dawley rats were fed American Institute of Nutrition 76 formula diet and were orally administrated KCL or acetic acid at a dose of 100 mg/kg body weight or deionized water for 4 weeks. Adipocyte size, DNA content in subcutaneous adipose tissue, lipid levels in the serum and liver, and the rate of fatty acid excretion were determined. Effects of KCL on pancreatic lipase activity and 3T3-L1 preadipocyte differentiation were investigated in vitro. Results. In the KCL group, the average adipocyte size in subcutaneous and perirenal adipose tissues was significantly reduced. The KCL-administered rats displayed greater numbers of small adipocytes in the subcutaneous, perirenal and mesenteric adipose tissues than did rats from the other groups. In the KCL group, the DNA content in subcutaneous adipose tissue was significantly increased. The rate of fatty acid excretion was significantly increased in the KCL group. Furthermore, KCL significantly inhibited pancreatic lipase activity in vitro, and also significantly inhibited fat accumulation and mRNA expression of fatty acid binding protein 2 (aP2) and peroxisome proliferator-activated (PPAR) in 3T3-L1 preadipocyte. The levels of serum and liver lipids, the concentration of serum glucose, and the levels of adiponectin were similar among the 3 groups. Conclusion. Oral administration of KCL decreases the adipocyte size via inhibition of dietary fat absorption and reductions of PPAR and aP2 mRNA expression levels in adipocytes.

Original languageEnglish
Article number134
JournalLipids in Health and Disease
Volume9
DOIs
Publication statusPublished - Nov 25 2010

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Adipocytes
Rats
Subcutaneous Fat
Peroxisome Proliferators
Liquids
Lipase
Fatty Acids
Serum
Tissue
Lipids
Formulated Food
Fatty Acid-Binding Proteins
Messenger RNA
Dietary Fats
Liver
Adiponectin
DNA
Nutrition
Hyperlipidemias
Acetic Acid

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical

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Effects of Kurozu concentrated liquid on adipocyte size in rats. / Tong, Li Tao; Katakura, Yoshinori; Kawamura, Sayaka; Baba, Sanae; Tanaka, Yasutake; Udono, Miyako; Kondo, Yoshie; Nakamura, Kumi; Imaizumi, Katsumi; Sato, Masao.

In: Lipids in Health and Disease, Vol. 9, 134, 25.11.2010.

Research output: Contribution to journalArticle

Tong, LT, Katakura, Y, Kawamura, S, Baba, S, Tanaka, Y, Udono, M, Kondo, Y, Nakamura, K, Imaizumi, K & Sato, M 2010, 'Effects of Kurozu concentrated liquid on adipocyte size in rats' Lipids in Health and Disease, vol. 9, 134. https://doi.org/10.1186/1476-511X-9-134
Tong, Li Tao ; Katakura, Yoshinori ; Kawamura, Sayaka ; Baba, Sanae ; Tanaka, Yasutake ; Udono, Miyako ; Kondo, Yoshie ; Nakamura, Kumi ; Imaizumi, Katsumi ; Sato, Masao. / Effects of Kurozu concentrated liquid on adipocyte size in rats. In: Lipids in Health and Disease. 2010 ; Vol. 9.
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abstract = "Background. Kurozu concentrated liquid (KCL) is used as a health-promoting supplement for the treatment of disorders such as cancer, hyperlipidemia, and hypertension in Japan. We investigated the possible anti-obesity effects of KCL in rats. Methods. Male Sprague Dawley rats were fed American Institute of Nutrition 76 formula diet and were orally administrated KCL or acetic acid at a dose of 100 mg/kg body weight or deionized water for 4 weeks. Adipocyte size, DNA content in subcutaneous adipose tissue, lipid levels in the serum and liver, and the rate of fatty acid excretion were determined. Effects of KCL on pancreatic lipase activity and 3T3-L1 preadipocyte differentiation were investigated in vitro. Results. In the KCL group, the average adipocyte size in subcutaneous and perirenal adipose tissues was significantly reduced. The KCL-administered rats displayed greater numbers of small adipocytes in the subcutaneous, perirenal and mesenteric adipose tissues than did rats from the other groups. In the KCL group, the DNA content in subcutaneous adipose tissue was significantly increased. The rate of fatty acid excretion was significantly increased in the KCL group. Furthermore, KCL significantly inhibited pancreatic lipase activity in vitro, and also significantly inhibited fat accumulation and mRNA expression of fatty acid binding protein 2 (aP2) and peroxisome proliferator-activated (PPAR) in 3T3-L1 preadipocyte. The levels of serum and liver lipids, the concentration of serum glucose, and the levels of adiponectin were similar among the 3 groups. Conclusion. Oral administration of KCL decreases the adipocyte size via inhibition of dietary fat absorption and reductions of PPAR and aP2 mRNA expression levels in adipocytes.",
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AU - Tong, Li Tao

AU - Katakura, Yoshinori

AU - Kawamura, Sayaka

AU - Baba, Sanae

AU - Tanaka, Yasutake

AU - Udono, Miyako

AU - Kondo, Yoshie

AU - Nakamura, Kumi

AU - Imaizumi, Katsumi

AU - Sato, Masao

PY - 2010/11/25

Y1 - 2010/11/25

N2 - Background. Kurozu concentrated liquid (KCL) is used as a health-promoting supplement for the treatment of disorders such as cancer, hyperlipidemia, and hypertension in Japan. We investigated the possible anti-obesity effects of KCL in rats. Methods. Male Sprague Dawley rats were fed American Institute of Nutrition 76 formula diet and were orally administrated KCL or acetic acid at a dose of 100 mg/kg body weight or deionized water for 4 weeks. Adipocyte size, DNA content in subcutaneous adipose tissue, lipid levels in the serum and liver, and the rate of fatty acid excretion were determined. Effects of KCL on pancreatic lipase activity and 3T3-L1 preadipocyte differentiation were investigated in vitro. Results. In the KCL group, the average adipocyte size in subcutaneous and perirenal adipose tissues was significantly reduced. The KCL-administered rats displayed greater numbers of small adipocytes in the subcutaneous, perirenal and mesenteric adipose tissues than did rats from the other groups. In the KCL group, the DNA content in subcutaneous adipose tissue was significantly increased. The rate of fatty acid excretion was significantly increased in the KCL group. Furthermore, KCL significantly inhibited pancreatic lipase activity in vitro, and also significantly inhibited fat accumulation and mRNA expression of fatty acid binding protein 2 (aP2) and peroxisome proliferator-activated (PPAR) in 3T3-L1 preadipocyte. The levels of serum and liver lipids, the concentration of serum glucose, and the levels of adiponectin were similar among the 3 groups. Conclusion. Oral administration of KCL decreases the adipocyte size via inhibition of dietary fat absorption and reductions of PPAR and aP2 mRNA expression levels in adipocytes.

AB - Background. Kurozu concentrated liquid (KCL) is used as a health-promoting supplement for the treatment of disorders such as cancer, hyperlipidemia, and hypertension in Japan. We investigated the possible anti-obesity effects of KCL in rats. Methods. Male Sprague Dawley rats were fed American Institute of Nutrition 76 formula diet and were orally administrated KCL or acetic acid at a dose of 100 mg/kg body weight or deionized water for 4 weeks. Adipocyte size, DNA content in subcutaneous adipose tissue, lipid levels in the serum and liver, and the rate of fatty acid excretion were determined. Effects of KCL on pancreatic lipase activity and 3T3-L1 preadipocyte differentiation were investigated in vitro. Results. In the KCL group, the average adipocyte size in subcutaneous and perirenal adipose tissues was significantly reduced. The KCL-administered rats displayed greater numbers of small adipocytes in the subcutaneous, perirenal and mesenteric adipose tissues than did rats from the other groups. In the KCL group, the DNA content in subcutaneous adipose tissue was significantly increased. The rate of fatty acid excretion was significantly increased in the KCL group. Furthermore, KCL significantly inhibited pancreatic lipase activity in vitro, and also significantly inhibited fat accumulation and mRNA expression of fatty acid binding protein 2 (aP2) and peroxisome proliferator-activated (PPAR) in 3T3-L1 preadipocyte. The levels of serum and liver lipids, the concentration of serum glucose, and the levels of adiponectin were similar among the 3 groups. Conclusion. Oral administration of KCL decreases the adipocyte size via inhibition of dietary fat absorption and reductions of PPAR and aP2 mRNA expression levels in adipocytes.

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