Effects of light and hydrogen peroxide on gene expression of newly identified antioxidant enzymes in the harmful algal bloom species Chattonella marina

Koki Mukai, Yohei Shimasaki, Xuchun Qiu, Yoko Kato-Unoki, Kun Chen, Mst Ruhina Margia Khanam, Yuji Oshima

Research output: Contribution to journalArticle

Abstract

Antioxidant enzymes are essential proteins that maintain cell proliferation potential by protecting against oxidative stress. They are present in many organisms including harmful algal bloom (HAB) species. We previously identified the antioxidant enzyme 2-Cys peroxiredoxin (PRX) in the raphidophyte Chattonella marina. This enzyme specifically decomposes a hydrogen peroxide (H2O2). PRX is the only antioxidant enzyme so far identified in C. marina. This study used mRNA-seq, using Trinity assemble and blastx for annotation, to identify a further five antioxidant enzymes from C. marina: Cu Zn superoxide dismutase (Cu/Zn-SOD), glutathione peroxidase (GPX), catalase (CAT), ascorbate peroxidase (APX) and thioredoxin (TRX). In the gene expression analysis of six enzymes (Cu/Zn-SOD, GPX, CAT, APX, TRX and PRX) using light-acclimated (100 μmol photons m−2 s−1) C. marina cells, only PRX gene expression levels were significantly increased by strong light irradiation (1000 μmol photons m−2 s−1). H2O2 concentration and scavenging activity were also increased and significantly positively correlated with PRX gene expression levels. In dark-acclimated cells, expression levels of all antioxidant enzymes except APX were significantly increased by light irradiation (100 μmol photons m−2 s−1). Expression decreased the following day, with the exception of PRX expression. With the exception of CAT, gene expression of antioxidant enzymes was not significantly induced by artificial H2O2 treatment, although average gene expression levels were slightly increased in some enzymes. Thus, we suggest that light is the main trigger of gene expression, but the resultant oxidative stress is also a possible factor affecting the gene expression of antioxidant enzymes in C. marina.

Original languageEnglish
Pages (from-to)393-403
Number of pages11
JournalEuropean Journal of Phycology
Volume54
Issue number3
DOIs
Publication statusPublished - Jan 1 2019

Fingerprint

marina
algal blooms
antioxidant
hydrogen peroxide
gene expression
peroxiredoxin
algal bloom
enzyme
antioxidants
enzymes
ascorbate peroxidase
catalase
glutathione peroxidase
superoxide dismutase
irradiation
oxidative stress
Chattonella
effect
cell proliferation
cells

All Science Journal Classification (ASJC) codes

  • Aquatic Science
  • Plant Science

Cite this

Effects of light and hydrogen peroxide on gene expression of newly identified antioxidant enzymes in the harmful algal bloom species Chattonella marina. / Mukai, Koki; Shimasaki, Yohei; Qiu, Xuchun; Kato-Unoki, Yoko; Chen, Kun; Khanam, Mst Ruhina Margia; Oshima, Yuji.

In: European Journal of Phycology, Vol. 54, No. 3, 01.01.2019, p. 393-403.

Research output: Contribution to journalArticle

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