Effects of low crystalline carbonate apatite on proliferation and osteoblastic differentiation of human bone marrow cells

Hirokazu Nagai, Masako Kobayashi-Fujioka, Kenji Fujisawa, Go Ohe, Natsumi Takamaru, Kanae Hara, Daisuke Uchida, Tetsuya Tamatani, Kunio Ishikawa, Youji Miyamoto

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Carbonated apatite (CO3Ap) is the inorganic component of bone. We have proposed a new method for the fabrication of CO3Ap blocks based on a dissolution-precipitation method using a synthetic precursor. The aim of this study is to examine the effects of low crystalline CO3Ap on initial cell attachment, proliferation and osteoblastic differentiation of human bone marrow cells (hBMCs) using sintered hydroxyapatite and tissue culture plates as controls. Initial cell attachment and proliferation were assessed with a MTT assay. Expression of osteoblastic markers was examined by reverse transcription-polymerase chain reaction. XRD and FT-IR results showed formation of B-type carbonate apatite with lower crystallinity. No difference was observed for initial cell attachment between HAp and CO3Ap discs. hBMSC attached more significantly on tissue culture plate than on HAp and CO3Ap discs. The number of cells on HAp was higher than that on CO3Ap until day 7, after which the number of cells was similar. hBMSC proliferated more significantly on tissue culture plate than on HAp and CO3Ap discs. In contrast, hBMCs incubated on CO3Ap demonstrated much higher expression of osteoblastic markers of differentiation, such as type I collagen, alkaline phosphatase, osteopontin and osteocalcin, than hBMCs on HAp. On the tissue culture plate, they were not any change throughout the culture period. These results demonstrated that low crystalline CO3Ap exhibit higher osteoinductivity than HAp.

Original languageEnglish
JournalJournal of Materials Science: Materials in Medicine
Volume26
Issue number2
DOIs
Publication statusPublished - Jan 1 2015

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Tissue culture
Apatite
Bone Marrow Cells
Carbonates
Bone
Cells
Crystalline materials
Cell Count
Cell Proliferation
Apatites
Osteopontin
Polymerase chain reaction
Osteocalcin
Differentiation Antigens
Phosphatases
Transcription
Durapatite
Collagen Type I
Hydroxyapatite
Collagen

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering

Cite this

Effects of low crystalline carbonate apatite on proliferation and osteoblastic differentiation of human bone marrow cells. / Nagai, Hirokazu; Kobayashi-Fujioka, Masako; Fujisawa, Kenji; Ohe, Go; Takamaru, Natsumi; Hara, Kanae; Uchida, Daisuke; Tamatani, Tetsuya; Ishikawa, Kunio; Miyamoto, Youji.

In: Journal of Materials Science: Materials in Medicine, Vol. 26, No. 2, 01.01.2015.

Research output: Contribution to journalArticle

Nagai, Hirokazu ; Kobayashi-Fujioka, Masako ; Fujisawa, Kenji ; Ohe, Go ; Takamaru, Natsumi ; Hara, Kanae ; Uchida, Daisuke ; Tamatani, Tetsuya ; Ishikawa, Kunio ; Miyamoto, Youji. / Effects of low crystalline carbonate apatite on proliferation and osteoblastic differentiation of human bone marrow cells. In: Journal of Materials Science: Materials in Medicine. 2015 ; Vol. 26, No. 2.
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