Effects of mechanical strain on differentiation of osteoblastic cells

Aritsune Matsui, Taisu L. Masuda, Takahisa Anada, Ichiro Takahashi, Fumihito Arai, Masamichi Takami, Ryutaro Kamijo, Toshio Fukuda, Seishi Echigo, Osamu Suzuki

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The present study was designed to investigate whether compressive mechanical strain affects osteoblastic cell differentiation. We developed a culture system with the multi channel that induces the mechanical stimuli with quantitative gradient strain on seeded cells during the culture. This culture system was a computer-driven device that the various mode of mechanical strains can be applied, using the pressurization or depressurization, to deform cells cultured on PDMS membrane. The mRNA expression levels of osteoblast-related genes, such as osterix, collagen type I, and osteopontin, determined by real- time PCR, decreased by the compressive strain. On the contrary, the expression of RANKL, a factor related to the induction of osteoclasts, increased in the compressive strain, while the expression of osteoprotegrin, a molecule that antagonizes the function of the RANKL, decreased in the same strain. It was suggested that the function of osteoblastic cells may be influenced by the compressive strain.

Original languageEnglish
Title of host publication2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics"
Pages475-480
Number of pages6
DOIs
Publication statusPublished - Dec 1 2008
Externally publishedYes
Event2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "System Cell Engineering by Multi-scale Manipulation" - Nagoya, Japan
Duration: Nov 6 2008Nov 9 2008

Publication series

Name2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008

Other

Other2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "System Cell Engineering by Multi-scale Manipulation"
CountryJapan
CityNagoya
Period11/6/0811/9/08

Fingerprint

Cells
Pressurization
Osteoblasts
Collagen
Genes
Membranes
Molecules

All Science Journal Classification (ASJC) codes

  • Computer Vision and Pattern Recognition
  • Human-Computer Interaction
  • Electrical and Electronic Engineering

Cite this

Matsui, A., Masuda, T. L., Anada, T., Takahashi, I., Arai, F., Takami, M., ... Suzuki, O. (2008). Effects of mechanical strain on differentiation of osteoblastic cells. In 2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics" (pp. 475-480). [4752499] (2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008). https://doi.org/10.1109/MHS.2008.4752499

Effects of mechanical strain on differentiation of osteoblastic cells. / Matsui, Aritsune; Masuda, Taisu L.; Anada, Takahisa; Takahashi, Ichiro; Arai, Fumihito; Takami, Masamichi; Kamijo, Ryutaro; Fukuda, Toshio; Echigo, Seishi; Suzuki, Osamu.

2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics". 2008. p. 475-480 4752499 (2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Matsui, A, Masuda, TL, Anada, T, Takahashi, I, Arai, F, Takami, M, Kamijo, R, Fukuda, T, Echigo, S & Suzuki, O 2008, Effects of mechanical strain on differentiation of osteoblastic cells. in 2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics"., 4752499, 2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, pp. 475-480, 2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "System Cell Engineering by Multi-scale Manipulation", Nagoya, Japan, 11/6/08. https://doi.org/10.1109/MHS.2008.4752499
Matsui A, Masuda TL, Anada T, Takahashi I, Arai F, Takami M et al. Effects of mechanical strain on differentiation of osteoblastic cells. In 2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics". 2008. p. 475-480. 4752499. (2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008). https://doi.org/10.1109/MHS.2008.4752499
Matsui, Aritsune ; Masuda, Taisu L. ; Anada, Takahisa ; Takahashi, Ichiro ; Arai, Fumihito ; Takami, Masamichi ; Kamijo, Ryutaro ; Fukuda, Toshio ; Echigo, Seishi ; Suzuki, Osamu. / Effects of mechanical strain on differentiation of osteoblastic cells. 2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics". 2008. pp. 475-480 (2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008).
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