Abstract
The present study was designed to investigate whether compressive mechanical strain affects osteoblastic cell differentiation. We developed a culture system with the multi channel that induces the mechanical stimuli with quantitative gradient strain on seeded cells during the culture. This culture system was a computer-driven device that the various mode of mechanical strains can be applied, using the pressurization or depressurization, to deform cells cultured on PDMS membrane. The mRNA expression levels of osteoblast-related genes, such as osterix, collagen type I, and osteopontin, determined by real- time PCR, decreased by the compressive strain. On the contrary, the expression of RANKL, a factor related to the induction of osteoclasts, increased in the compressive strain, while the expression of osteoprotegrin, a molecule that antagonizes the function of the RANKL, decreased in the same strain. It was suggested that the function of osteoblastic cells may be influenced by the compressive strain.
| Original language | English |
|---|---|
| Title of host publication | 2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics" |
| Pages | 475-480 |
| Number of pages | 6 |
| DOIs | |
| Publication status | Published - Dec 1 2008 |
| Externally published | Yes |
| Event | 2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "System Cell Engineering by Multi-scale Manipulation" - Nagoya, Japan Duration: Nov 6 2008 → Nov 9 2008 |
Publication series
| Name | 2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008 |
|---|
Other
| Other | 2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "System Cell Engineering by Multi-scale Manipulation" |
|---|---|
| Country | Japan |
| City | Nagoya |
| Period | 11/6/08 → 11/9/08 |
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All Science Journal Classification (ASJC) codes
- Computer Vision and Pattern Recognition
- Human-Computer Interaction
- Electrical and Electronic Engineering
Cite this
Effects of mechanical strain on differentiation of osteoblastic cells. / Matsui, Aritsune; Masuda, Taisu L.; Anada, Takahisa; Takahashi, Ichiro; Arai, Fumihito; Takami, Masamichi; Kamijo, Ryutaro; Fukuda, Toshio; Echigo, Seishi; Suzuki, Osamu.
2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics". 2008. p. 475-480 4752499 (2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Effects of mechanical strain on differentiation of osteoblastic cells
AU - Matsui, Aritsune
AU - Masuda, Taisu L.
AU - Anada, Takahisa
AU - Takahashi, Ichiro
AU - Arai, Fumihito
AU - Takami, Masamichi
AU - Kamijo, Ryutaro
AU - Fukuda, Toshio
AU - Echigo, Seishi
AU - Suzuki, Osamu
PY - 2008/12/1
Y1 - 2008/12/1
N2 - The present study was designed to investigate whether compressive mechanical strain affects osteoblastic cell differentiation. We developed a culture system with the multi channel that induces the mechanical stimuli with quantitative gradient strain on seeded cells during the culture. This culture system was a computer-driven device that the various mode of mechanical strains can be applied, using the pressurization or depressurization, to deform cells cultured on PDMS membrane. The mRNA expression levels of osteoblast-related genes, such as osterix, collagen type I, and osteopontin, determined by real- time PCR, decreased by the compressive strain. On the contrary, the expression of RANKL, a factor related to the induction of osteoclasts, increased in the compressive strain, while the expression of osteoprotegrin, a molecule that antagonizes the function of the RANKL, decreased in the same strain. It was suggested that the function of osteoblastic cells may be influenced by the compressive strain.
AB - The present study was designed to investigate whether compressive mechanical strain affects osteoblastic cell differentiation. We developed a culture system with the multi channel that induces the mechanical stimuli with quantitative gradient strain on seeded cells during the culture. This culture system was a computer-driven device that the various mode of mechanical strains can be applied, using the pressurization or depressurization, to deform cells cultured on PDMS membrane. The mRNA expression levels of osteoblast-related genes, such as osterix, collagen type I, and osteopontin, determined by real- time PCR, decreased by the compressive strain. On the contrary, the expression of RANKL, a factor related to the induction of osteoclasts, increased in the compressive strain, while the expression of osteoprotegrin, a molecule that antagonizes the function of the RANKL, decreased in the same strain. It was suggested that the function of osteoblastic cells may be influenced by the compressive strain.
UR - http://www.scopus.com/inward/record.url?scp=62449245786&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=62449245786&partnerID=8YFLogxK
U2 - 10.1109/MHS.2008.4752499
DO - 10.1109/MHS.2008.4752499
M3 - Conference contribution
AN - SCOPUS:62449245786
SN - 9781424429196
T3 - 2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008
SP - 475
EP - 480
BT - 2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics"
ER -