TY - GEN
T1 - Effects of mechanical stress and scaffold material on osteogenesis and chondrogenesis
AU - Suzuki, O.
AU - Takahashi, I.
AU - Kamakura, S.
AU - Sasaki, K.
AU - Kamijo, R.
AU - Nakamura, M.
AU - Oda, M.
AU - Uchida, T.
AU - Arai, F.
AU - Fukuda, T.
PY - 2005
Y1 - 2005
N2 - The present study was designed to investigate how mouse bone marrow stromal ST2 cells and rat limb mesenchymal stem cells respond to mechanical stress. Mechanical stress loading culture devices originally developed or commercially available were used to induce quantitative strain to these cells. Differentiation of ST2, estimated by alkaline phosphatase (ALP), was shown to be affected by the amount of the strain. Chondrogenic cell differentiation was enhanced by stretch stimulation through phosphplylation of extracellular- signaling regulated kinases (ERKs). In this study, effect of synthetic scaffold octacalcium phosphate (OCP) was also determined. OCP implantation enhanced bone regeneration. The results suggest that the differentiation of these skeletal tissue forming cells is under control of the mechanical stresses through the signaling cascade. It is likely that the scaffold plays a role of site scaffolding the cells to assist differentiation.
AB - The present study was designed to investigate how mouse bone marrow stromal ST2 cells and rat limb mesenchymal stem cells respond to mechanical stress. Mechanical stress loading culture devices originally developed or commercially available were used to induce quantitative strain to these cells. Differentiation of ST2, estimated by alkaline phosphatase (ALP), was shown to be affected by the amount of the strain. Chondrogenic cell differentiation was enhanced by stretch stimulation through phosphplylation of extracellular- signaling regulated kinases (ERKs). In this study, effect of synthetic scaffold octacalcium phosphate (OCP) was also determined. OCP implantation enhanced bone regeneration. The results suggest that the differentiation of these skeletal tissue forming cells is under control of the mechanical stresses through the signaling cascade. It is likely that the scaffold plays a role of site scaffolding the cells to assist differentiation.
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U2 - 10.1109/MHS.2005.1589996
DO - 10.1109/MHS.2005.1589996
M3 - Conference contribution
AN - SCOPUS:33847009472
SN - 0780394828
SN - 9780780394827
T3 - Proceedings of the 2005 International Symposium on Micro-NanoMechatronics and Human Science, Eighth Symposium on Micro- and Nano-Mechatronics for Information-Based Society - The 21st Century COE Progr
SP - 235
EP - 240
BT - Proceedings of the 2005 International Symposium on Micro-NanoMechatronics and Human Science, Eighth Symposium on Micro- and Nano-Mechatronics for Information-Based Society - The 21st Century COE Progr
T2 - 2005 International Symposium on Micro-NanoMechatronics and Human Science, Eighth Symposium on Micro- and Nano-Mechatronics for Information-Based Society - The 21st Century COE Program
Y2 - 7 November 2005 through 9 November 2005
ER -