Effects of missense mutations Phe110Ile and Glu244Asp in human cardiac troponin T on force generation in skinned cardiac muscle fibers

Hiroyuki Nakaura, Fumi Yanaga, Iwao Ohtsuki, Sachio Morimoto

Research output: Contribution to journalArticlepeer-review

37 Citations (Scopus)

Abstract

The functional effects of two missense mutations in human cardiac troponin T, Phe110Ile and Glu244Asp, associated with familial hypertrophic cardiomyopathy were examined by exchanging the bacterially expressed and purified mutant troponin T into rabbit cardiac skinned muscle fibers. Both mutations significantly increased the maximum force without affecting the cooperativity. The Glu244Asp mutation also increased the Ca2+ sensitivity of the force generation, as in the case of other mutations associated with a poor prognosis. On the other hand, the Phe110Ile mutation, associated with a favorable prognosis, had no effect on the Ca2+ sensitivity. The results strongly support the hypothesis that increased Ca2+ sensitivity is responsible for the pathogenesis of hypertrophic cardiomyopathy with a poor prognosis caused by mutations in troponin T.

Original languageEnglish
Pages (from-to)457-460
Number of pages4
JournalJournal of biochemistry
Volume126
Issue number3
DOIs
Publication statusPublished - Jan 1 1999

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

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