Effects of monocrotaline pyrrole on morphology, number and production of nitric oxide of cultured artery endothelial cells

W. Cheng, Z. Li, T. Koyama, Masahiro Oike, Y. Ito

Research output: Contribution to journalArticle

Abstract

Experimental model of pulmonary hypertension has been created by exposing animals to monocrotaline pyrrole (MCTP), but the cause of pulmonary hypertension induced by MCTP is still controversial. In this study, cell culture and DAF-2 fluorescence technique were used to investigate the effects of MCTP on Cell surface area, cell number and production of nitric oxide (NO) in cultured calf pulmonary artery endothelial cells (CPAE) and bovine aorta . endothelial cells (BAEC). MCTP induced the cell enlargement from (46 ± 4) to (223 ± 27) μm2 and decreased the number of cells from (175 ± 9) × 106 L-1 to (49 ± 6) × 106 L-1 in CPAE. Acetylcholine induced production of NO, measured by DAF-2, was decreased significantly from (16.1 ± 1.3) % to (1.5 ± 1.1)% and from (14.2 ± 1.6)% to (1.4 ± 1.6) % in CPAE and BAEC, respectively. The results indicate that MCTP may damage endothelial cells and inhibit NO production, which maybe another pathogenesis of MCTP induced pulmonary hypertension.

Original languageEnglish
Pages (from-to)169-171
Number of pages3
JournalChinese Journal of Pharmacology and Toxicology
Volume15
Issue number3
Publication statusPublished - Jan 1 2001
Externally publishedYes

Fingerprint

Endothelial cells
Nitric Oxide
Endothelial Cells
Arteries
Pulmonary Hypertension
Pulmonary Artery
Cell Count
Cell Enlargement
Cell culture
Acetylcholine
Aorta
monocrotaline pyrrole
Animals
Theoretical Models
Cell Culture Techniques
Fluorescence

All Science Journal Classification (ASJC) codes

  • Toxicology
  • Pharmacology

Cite this

Effects of monocrotaline pyrrole on morphology, number and production of nitric oxide of cultured artery endothelial cells. / Cheng, W.; Li, Z.; Koyama, T.; Oike, Masahiro; Ito, Y.

In: Chinese Journal of Pharmacology and Toxicology, Vol. 15, No. 3, 01.01.2001, p. 169-171.

Research output: Contribution to journalArticle

@article{96ca1c12af794dae854b2c39fdcc0184,
title = "Effects of monocrotaline pyrrole on morphology, number and production of nitric oxide of cultured artery endothelial cells",
abstract = "Experimental model of pulmonary hypertension has been created by exposing animals to monocrotaline pyrrole (MCTP), but the cause of pulmonary hypertension induced by MCTP is still controversial. In this study, cell culture and DAF-2 fluorescence technique were used to investigate the effects of MCTP on Cell surface area, cell number and production of nitric oxide (NO) in cultured calf pulmonary artery endothelial cells (CPAE) and bovine aorta . endothelial cells (BAEC). MCTP induced the cell enlargement from (46 ± 4) to (223 ± 27) μm2 and decreased the number of cells from (175 ± 9) × 106 L-1 to (49 ± 6) × 106 L-1 in CPAE. Acetylcholine induced production of NO, measured by DAF-2, was decreased significantly from (16.1 ± 1.3) {\%} to (1.5 ± 1.1){\%} and from (14.2 ± 1.6){\%} to (1.4 ± 1.6) {\%} in CPAE and BAEC, respectively. The results indicate that MCTP may damage endothelial cells and inhibit NO production, which maybe another pathogenesis of MCTP induced pulmonary hypertension.",
author = "W. Cheng and Z. Li and T. Koyama and Masahiro Oike and Y. Ito",
year = "2001",
month = "1",
day = "1",
language = "English",
volume = "15",
pages = "169--171",
journal = "Chinese Journal of Pharmacology and Toxicology",
issn = "1000-3002",
publisher = "Chinese Journal of Pharmacology and Toxicology",
number = "3",

}

TY - JOUR

T1 - Effects of monocrotaline pyrrole on morphology, number and production of nitric oxide of cultured artery endothelial cells

AU - Cheng, W.

AU - Li, Z.

AU - Koyama, T.

AU - Oike, Masahiro

AU - Ito, Y.

PY - 2001/1/1

Y1 - 2001/1/1

N2 - Experimental model of pulmonary hypertension has been created by exposing animals to monocrotaline pyrrole (MCTP), but the cause of pulmonary hypertension induced by MCTP is still controversial. In this study, cell culture and DAF-2 fluorescence technique were used to investigate the effects of MCTP on Cell surface area, cell number and production of nitric oxide (NO) in cultured calf pulmonary artery endothelial cells (CPAE) and bovine aorta . endothelial cells (BAEC). MCTP induced the cell enlargement from (46 ± 4) to (223 ± 27) μm2 and decreased the number of cells from (175 ± 9) × 106 L-1 to (49 ± 6) × 106 L-1 in CPAE. Acetylcholine induced production of NO, measured by DAF-2, was decreased significantly from (16.1 ± 1.3) % to (1.5 ± 1.1)% and from (14.2 ± 1.6)% to (1.4 ± 1.6) % in CPAE and BAEC, respectively. The results indicate that MCTP may damage endothelial cells and inhibit NO production, which maybe another pathogenesis of MCTP induced pulmonary hypertension.

AB - Experimental model of pulmonary hypertension has been created by exposing animals to monocrotaline pyrrole (MCTP), but the cause of pulmonary hypertension induced by MCTP is still controversial. In this study, cell culture and DAF-2 fluorescence technique were used to investigate the effects of MCTP on Cell surface area, cell number and production of nitric oxide (NO) in cultured calf pulmonary artery endothelial cells (CPAE) and bovine aorta . endothelial cells (BAEC). MCTP induced the cell enlargement from (46 ± 4) to (223 ± 27) μm2 and decreased the number of cells from (175 ± 9) × 106 L-1 to (49 ± 6) × 106 L-1 in CPAE. Acetylcholine induced production of NO, measured by DAF-2, was decreased significantly from (16.1 ± 1.3) % to (1.5 ± 1.1)% and from (14.2 ± 1.6)% to (1.4 ± 1.6) % in CPAE and BAEC, respectively. The results indicate that MCTP may damage endothelial cells and inhibit NO production, which maybe another pathogenesis of MCTP induced pulmonary hypertension.

UR - http://www.scopus.com/inward/record.url?scp=0034951593&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034951593&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0034951593

VL - 15

SP - 169

EP - 171

JO - Chinese Journal of Pharmacology and Toxicology

JF - Chinese Journal of Pharmacology and Toxicology

SN - 1000-3002

IS - 3

ER -