TY - JOUR
T1 - Effects of nanoparticle size and cell type on high sensitivity cell detection using a localized surface plasmon resonance biosensor
AU - Liu, Fei
AU - Wong, Matthew Man Kin
AU - Chiu, Sung Kay
AU - Lin, Hao
AU - Ho, Johnny C.
AU - Pang, Stella W.
N1 - Funding Information:
This work was supported by the Center for Biosystems, Neuroscience, and Nanotechnology of City University of Hong Kong under Project number 9360148 . We gratefully acknowledge Dr. Qing Yuan Tang, Miss Tsing Chung, Dr. Polis Wong, Mr. Robust Lai, Dr. Payton Lin, Dr. Shang Xin Lin, Mr. Bing Zou, and Mr. Michael Chiang for their technical support and helpful discussions.
PY - 2014/5/15
Y1 - 2014/5/15
N2 - A localized surface plasmon resonance (LSPR) effect was used to distinguish cell concentration on ordered arrays of Au nanoparticles (NPs) on glass substrates. Human-derived retinal pigment epithelial RPE-1 cells with flatter bodies and higher confluency were compared with breast cancer MCF-7 cells. Nanosphere lithography was used to form Au NPs with average diameters of 500 and 60nm in order to compare cell detection range, resonance peak shift, and cell concentration sensitivity. A larger cell concentration range was detected on the larger 500nm Au NPs compared to 60nm Au NPs (8.56×103-1.09×106 vs. 3.43×104-2.73×105cells/ml). Resonance peak shift could distinguish RPE-1 from MCF-7 cells on both Au NPs. RPE-1 cells consistently displayed larger resonance peak shifts compared to MCF-7 cells until the detection became saturated at higher concentration. For both types of cells, higher concentration sensitivity in the range of ~104-106cells/ml was observed on 500nm compared to 60nm Au NPs. Our results show that cells on Au NPs can be detected in a large range and at low concentration. Optimal cell sensing can be achieved by altering the dimensions of Au NPs according to different cell characteristics and concentrations.
AB - A localized surface plasmon resonance (LSPR) effect was used to distinguish cell concentration on ordered arrays of Au nanoparticles (NPs) on glass substrates. Human-derived retinal pigment epithelial RPE-1 cells with flatter bodies and higher confluency were compared with breast cancer MCF-7 cells. Nanosphere lithography was used to form Au NPs with average diameters of 500 and 60nm in order to compare cell detection range, resonance peak shift, and cell concentration sensitivity. A larger cell concentration range was detected on the larger 500nm Au NPs compared to 60nm Au NPs (8.56×103-1.09×106 vs. 3.43×104-2.73×105cells/ml). Resonance peak shift could distinguish RPE-1 from MCF-7 cells on both Au NPs. RPE-1 cells consistently displayed larger resonance peak shifts compared to MCF-7 cells until the detection became saturated at higher concentration. For both types of cells, higher concentration sensitivity in the range of ~104-106cells/ml was observed on 500nm compared to 60nm Au NPs. Our results show that cells on Au NPs can be detected in a large range and at low concentration. Optimal cell sensing can be achieved by altering the dimensions of Au NPs according to different cell characteristics and concentrations.
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U2 - 10.1016/j.bios.2013.11.075
DO - 10.1016/j.bios.2013.11.075
M3 - Article
C2 - 24373953
AN - SCOPUS:84891052548
SN - 0956-5663
VL - 55
SP - 141
EP - 148
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
ER -
