Effects of nanoparticle size and cell type on high sensitivity cell detection using a localized surface plasmon resonance biosensor

Fei Liu, Matthew Man Kin Wong, Sung Kay Chiu, Hao Lin, Johnny C. Ho, Stella W. Pang

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)

Abstract

A localized surface plasmon resonance (LSPR) effect was used to distinguish cell concentration on ordered arrays of Au nanoparticles (NPs) on glass substrates. Human-derived retinal pigment epithelial RPE-1 cells with flatter bodies and higher confluency were compared with breast cancer MCF-7 cells. Nanosphere lithography was used to form Au NPs with average diameters of 500 and 60nm in order to compare cell detection range, resonance peak shift, and cell concentration sensitivity. A larger cell concentration range was detected on the larger 500nm Au NPs compared to 60nm Au NPs (8.56×103-1.09×106 vs. 3.43×104-2.73×105cells/ml). Resonance peak shift could distinguish RPE-1 from MCF-7 cells on both Au NPs. RPE-1 cells consistently displayed larger resonance peak shifts compared to MCF-7 cells until the detection became saturated at higher concentration. For both types of cells, higher concentration sensitivity in the range of ~104-106cells/ml was observed on 500nm compared to 60nm Au NPs. Our results show that cells on Au NPs can be detected in a large range and at low concentration. Optimal cell sensing can be achieved by altering the dimensions of Au NPs according to different cell characteristics and concentrations.

Original languageEnglish
Pages (from-to)141-148
Number of pages8
JournalBiosensors and Bioelectronics
Volume55
DOIs
Publication statusPublished - May 15 2014
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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