Effects of overexpression of PTP36, a putative protein tyrosine phosphatase, on cell adhesion, cell growth, and cytoskeletons in HeLa cells

Masato Ogata, Tsuyoshi Takada, Yoshiko Mori, Masatsugu Oh-Hora, Yohzo Uchida, Atsushi Kosugi, Kensuke Miyake, Toshiyuki Hamaoka

    Research output: Contribution to journalArticle

    33 Citations (Scopus)

    Abstract

    Non-receptor-type putative protein tyrosine phosphatase-36 (PTP36), also known as PTPD2/Pez, possesses a domain homologous to the N-terminal half of band 4.1 protein. To gain insight into the biological function of PTP36, we established a HeLa cell line, HtTA/P36-9, in which the overexpression of PTP36 was inducible. PTP36 expressed in HeLa cells was enriched in the cytoskeleton near the plasma membrane. There was little endogenous PTP36 detectable in uninduced HtTA/P36-9 cells or in the parental HeLa cells. Upon induction of PTP36 overexpression, HtTA/P36-9 cells spread less well, grew more slowly, and adhered to the extracellular matrix proteins less well than uninduced cells. Moreover, decreases in the actin stress fibers and the number of focal adhesions were observed. The tyrosine phosphorylation of the focal adhesion kinase induced by lysophosphatidic acid was suppressed in the HtTA/P36-9 cells overexpressing PTP36. These results indicate that PTP36 affects cytoskeletons, cell adhesion, and cell growth, thus suggesting that PTP36 is involved in their regulatory processes.

    Original languageEnglish
    Pages (from-to)12905-12909
    Number of pages5
    JournalJournal of Biological Chemistry
    Volume274
    Issue number18
    DOIs
    Publication statusPublished - Apr 30 1999

    Fingerprint

    Protein Tyrosine Phosphatases
    Cell adhesion
    Cell growth
    Cytoskeleton
    HeLa Cells
    Cell Adhesion
    Growth
    Non-Receptor Protein Tyrosine Phosphatases
    Focal Adhesion Protein-Tyrosine Kinases
    Stress Fibers
    Focal Adhesions
    Extracellular Matrix Proteins
    Phosphorylation
    Cell membranes
    Tyrosine
    Actins
    Cell Membrane
    Adhesion
    Cells
    Cell Line

    All Science Journal Classification (ASJC) codes

    • Biochemistry
    • Molecular Biology
    • Cell Biology

    Cite this

    Effects of overexpression of PTP36, a putative protein tyrosine phosphatase, on cell adhesion, cell growth, and cytoskeletons in HeLa cells. / Ogata, Masato; Takada, Tsuyoshi; Mori, Yoshiko; Oh-Hora, Masatsugu; Uchida, Yohzo; Kosugi, Atsushi; Miyake, Kensuke; Hamaoka, Toshiyuki.

    In: Journal of Biological Chemistry, Vol. 274, No. 18, 30.04.1999, p. 12905-12909.

    Research output: Contribution to journalArticle

    Ogata, Masato ; Takada, Tsuyoshi ; Mori, Yoshiko ; Oh-Hora, Masatsugu ; Uchida, Yohzo ; Kosugi, Atsushi ; Miyake, Kensuke ; Hamaoka, Toshiyuki. / Effects of overexpression of PTP36, a putative protein tyrosine phosphatase, on cell adhesion, cell growth, and cytoskeletons in HeLa cells. In: Journal of Biological Chemistry. 1999 ; Vol. 274, No. 18. pp. 12905-12909.
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    abstract = "Non-receptor-type putative protein tyrosine phosphatase-36 (PTP36), also known as PTPD2/Pez, possesses a domain homologous to the N-terminal half of band 4.1 protein. To gain insight into the biological function of PTP36, we established a HeLa cell line, HtTA/P36-9, in which the overexpression of PTP36 was inducible. PTP36 expressed in HeLa cells was enriched in the cytoskeleton near the plasma membrane. There was little endogenous PTP36 detectable in uninduced HtTA/P36-9 cells or in the parental HeLa cells. Upon induction of PTP36 overexpression, HtTA/P36-9 cells spread less well, grew more slowly, and adhered to the extracellular matrix proteins less well than uninduced cells. Moreover, decreases in the actin stress fibers and the number of focal adhesions were observed. The tyrosine phosphorylation of the focal adhesion kinase induced by lysophosphatidic acid was suppressed in the HtTA/P36-9 cells overexpressing PTP36. These results indicate that PTP36 affects cytoskeletons, cell adhesion, and cell growth, thus suggesting that PTP36 is involved in their regulatory processes.",
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    AU - Oh-Hora, Masatsugu

    AU - Uchida, Yohzo

    AU - Kosugi, Atsushi

    AU - Miyake, Kensuke

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